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Western blot analysis of adult mouse brain.  The blot was probed with rabbit polyclonal anti-SCAI (N-terminal region) antibody in the presence (lanes 2) or absence (lane 1) of SCAI (N-terminal region) blocking peptide (SX3845), or unrelated peptide (lane 3).
Cultured hippocampal neurons (9 DIV) double- Stained for MAP2 (red), L5/1 (green).Adult rat neocortex immunohistochemistry.
Western blot image of human A431 cells and (lane 1) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lane 2). The blots were probed with rabbit polyclonal anti-Semaphorin 3A (SP1241) at a dilution of 1:1000.
Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Sema3A (SP1401) (lanes 1 & 2), anti-Sema3A (SP1221) (lanes 3 & 4) and anti-Sema3A (SP1241) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Sema3A.Immunocytochemical labeling of Sema-3A in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal Sema-3A (N-terminal) antibody (SP1401), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

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