Anti-Nav1.7 Na+ Channel Antibody (N68/6)

Name: Anti-Nav1.7 Na+ Channel Antibody
Brand: NeuroMab
SKU: 75-103
Price: 394.0 USD
Availability: InStock

Our mouse Nav1.7 Na+ channel monoclonal antibody from NeuroMab is produced in-house from clone N68/6. It detects human, mouse, non-human primate, and rat Nav1.7 Na+ channel and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and HEK-293 cells stably expressing Nav1.3, and Nav1.7 and transiently expressing Kv2.1 probed with N68/6 (left) or K58/35 (right) TC supe.

Immunostaining of olfactory bulb and olfactory epithelium of normosmic mice. The 4-um section PPFE tissue was deparffinizeed with EZPrep buffer. For IHC, immunostaining was performed using Anti-NaV1.7 (Cat. 75-103, DAB-brown, 0.5 ug/ml) and counterstained with hematoxylin (blue). Image from publication, CC-BY-4.0. PMID: 38960711.

Induced pluripotent stem cell-derived sensory neurons (iPSC-SNs) stained positively for peripherin (a marker of peripheral neurons), Brn3a (a marker of sensory neurons48), NeuN (a marker of neuronal nuclei), and NaV1.7 (Cat. 75-103, 1:250). Image from publication CC-BY-4.0. PMID:34930944

Immunofluorescent micrographs showing Fluoro-gold-traced joint afferents (blue) and Nav1.7 labelled cells (green) in (A) naïve, (B) MIA, (C) LPA, and (D) MMT rats. Yellow arrows indicate joint afferents that are negative for Nav1.7 and white arrows point to Fluoro-gold positive joint afferents that co-express Nav1.7. Image from publication CC-BY-4.0. PMID:36358921

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and HEK-293 cells stably expressing Nav1.3, and Nav1.7 and transiently expressing Kv2.1 probed with N68/6 (left) or K58/35 (right) TC supe.

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Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-103

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Nav1.7 Na+ channel

Scientific Background

Nav1.7 subunit alpha, or SCN9A, is a member of the voltage gated sodium channel family. It is expressed at the terminal ends of sensory neurons in the dorsal root ganglion (DRG) and in trigeminal and sympathetic ganglion neurons. A major function of Nav1.7 is the generation and conduction of action potentials in nociceptors, or “pain receptors” and thus involved in pain sensation. Changes in Nav1.7 expression have been linked to primary erythermalgia, primary erythermalgia, and Congenital insensitivity to pain

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N68/6

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

SCN9A NENA

Molecular Weight

230 kDa

Immunogen

Fusion protein amino acids 1751-1946 (cytoplasmic C-terminus) of human Nav1.7 (accession number Q15858) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2184355

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.31

Dilution Ranges

WB: 1:500
IHC: 1:250
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity against other Nav channels

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Sodium channel protein type 9 subunit alpha (Neuroendocrine sodium channel) (hNE-Na) (Peripheral sodium channel 1) (PN1) (Sodium channel protein type IX subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.7)

UniProt ID

Q15858

Shipping

Shipped on ice packs

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Anti-Nav1.7 Na+ Channel Antibody (N68/6)

SKU: 75-103

Product Details

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