Polyclonal Antibodies

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Immunohistochemistry of mouse inner ear frozen sections showing specific staining of GLUT10 at 1:200 dilution. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.Western Blot showing specific immunolabeling of GLUT10 in Lane 1: 10ug of protein from liver Lane 2: 10ug of protein from cochlea (E18) Lane 3: 10ug of protein from cochlea (P7) Lane 4: 10ug of protein from cochlea (P10) Lane 5: 10ug of protein from cochlea (P14) Lane 6: 10ug of protein from cochlea (P17) Lane 7: 10ug of protein from cochlea (P60) Lane 8: 10ug of protein from cochlea (1 Year).
Western Blot of GST-hSAP30L fusion protein in NIH3T3 cells (Lane 1), wild-type transfected hSAP30L (Lane 2), and endogenous hSAP30L in non-transfected MCF-7 cells showing specific immunolabeling of SAP30L.
Western Blot of HEK293 cells with DJ-1/V5 fusion, and mouse brain lysates showing specific immunolabeling of Park7(DJ-1).
Immunoprecipitation showing detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.Western Blot of 50 ug lysates of A431 (lane 1) and AR42J (lane 2) cells showing specific immunolabeling of Ku80.
Immunoprecipitation of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.Western blot of T47D cells (left) and HeLa cells (right) showing specific immunolabeling of the ~80 kDa Ku protein at a dilution of 1:500.
Western Blot of 20 ug of human lung adenocarcinoma cell line NCI-H322 lysate showing specific immunolabeling of Kindlin.
Western Blot of 100 ng of Rattin peptide and 50 ug of 293 T cell lysates transfected with plasmids encoding GFP-Rattin showing specific immunolabeling of Rattin.
Western Blot of 100 ng of synthetic Humanin peptide and 50 ug of 293 T cell lysates transfected with plasmids encoding GFPc1 or GFP-Humanin showing specific immunolabeling of Humanin.
Western Blot of 30 ug of recombinant human full-length FBG4 showing specific immunolabeling of FBG4.
Western Blot of 30 ug of recombinant human full length FBG3 protein showing specific immunolabeling of FBG3.
Western Blot of 50ug of human heart lysate showing specific immunolabeling of PRG3.
Western Blot of 293 T cell lysate overexpressed with NORE1 showing specific immunolabeling of NORE1.
Western Blot of 30ug HepG2 cell lysate showing specific immunolabeling of CIDE3.
Western Blot of 50 ug of mouse brain lysate showing specific immunolabeling of SHPRH.
Immunohistochemistry of the neocortex of Alzheimer's diseased brain sections (floating, fixed with 10% formalin for 24 hrs) showing specific staining of Collagen XXV. Western Blot of Lane 1: full-length CLAC-P (lysate), Lane 2: secreted form of CLAC-P (media), Lane 3: COLXIII (lysate), Lane 4 degrees COLXIII (media), Lane 5: COLXXIII (lysate), Lane 6: COLXXIII (media) showing specific immunolabeling of Collagen XXV alpha 1.
Western Blot of 100 ug of formic acid extracts of Alzheimer diseased brain showing specific immunolabeling of Collagen XXV.

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