Anti-Vimentin Antibody

Name: Anti-Vimentin Antibody
Brand: Biosensis
SKU: R-1699-100
Price: 337.0 USD
Availability: InStock

Our Anti-Vimentin rabbit polyclonal primary antibody detects human, mouse, other mammals and (predicted) Vimentin, and is whole serum. It is validated for use in ICC, WB.

Western blot of crude extract of HeLa cells showing a single strong clean band at about 55 kDa.

HeLa cells stained with Rabbit anti-Vimentin (R-1699-100) (red) and counterstained with Monoclonal antibody to Lamin A/C (green, M-1689-100). DNA is blue. R-1699-100 antibody reveals strong cytoplasmic intermediate filament staining, while M-1689-100 antibody reveals strong nuclear lamina staining.

Left: Analysis of vimentin expression in HeLa cells by Immunocytochemistry with rabbit antibody to vimentin (green, 1:5,000). Cells were co-stained with an antibody to actin (red). Blue: DAPI nuclear stain. The vimentin antibody stains the 10 nm or intermediate filament network of the cytoskeleton. Right: Western blot analysis of whole cell lysates using rabbit antibody to vimentin (green, 1:5,000). [1] protein standard, [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3. The strong band at around 55 kDa corresponds to vimentin protein.

Western blot of crude extract of HeLa cells showing a single strong clean band at about 55 kDa.

Click on image to zoom

Species Reactivity

Human, Mouse

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1699-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Vimentin

Scientific Background

Vimentin is the major protein subunit of the 10 nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells. Vimentin is also found in many kinds of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton.

Format

Whole serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Molecular Weight

55 kDa

Immunogen

Recombinant human Vimentin purified from E. coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized with sodium azide.

Dilution Ranges

WB: 1:5000-1:10000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Specific for the ~55 kDa Vimentin protein.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; Vimentin

UniProt ID

P08670
UniProt Summary: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.

NCBI Gene ID

7431

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Anti-Vimentin Antibody

SKU: R-1699-100

Product Details

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