Anti-Ubiquitin Antibody

Name: Anti-Ubiquitin Antibody
Brand: Biosensis
SKU: R-182-250
Price: 347.0 USD
Availability: InStock

Our Anti-Ubiquitin rabbit polyclonal primary antibody detects bovine, human, other mammals (predicted), non-human primate, and rat Ubiquitin, and is IgG. It is validated for use in ICC, IHC-Frozen, WB.

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Species Reactivity

Bovine, Human, Monkey, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-182-250

Volume/Size: 250 µg

Variant

Price:

$347.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ubiquitin

Scientific Background

Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Immunogen

Ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins with glutaraldehyde.

Immunogen Species

Bovine

Species Reactivity

Bovine, Human, Monkey, Rat

Storage

Spin vial briefly before opening. Reconstitute in 250 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified immunoglobulin.

Buffer

Lyophilized from PBS, pH 7.2, 100mM NaCl, 15mM sodium azide.

Dilution Ranges

IHC: 1:150-1:300

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been determined by indirect ELISA against ubiquitin conjugated to keyhole limpet haemocyanin. The antibody does not react with kehole limpet haemocyanin alone. Specificity has also been demonstrated by WB against endometrial tissue homogenates. Cross reacts with human, baboon and rat ubiquitin. Other species have not been tested. Ubiquitin protein sequence is 100% conserved in all higher mammals and most eukarotes.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

polyubiquitin-B; RPS27A; UBA52; UBB; UBC

UniProt ID

P0CG53
UniProt Summary: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

NCBI Gene ID

281370

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Anti-Ubiquitin Antibody

SKU: R-182-250

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