Anti-GABA-A-R, Beta1 Antibody (N96/55)

Name: Anti-GABA-A-R, Beta1 Antibody
Brand: NeuroMab
SKU: 75-137
Price: 337.0 USD
Availability: InStock

Our mouse GABA(A)R, Beta1 monoclonal antibody from NeuroMab is produced in-house from clone N96/55. It detects human, mouse, and rat GABA(A)R, Beta1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Adult rat hippocampus immunohistochemistry.

Immunofluorescence of cultured hippocampal neurons with N96/55 (red), GABA- A-R-Gamma2 rabbit polyclonal (green) and GAD (blue). Images courtesy of Angel de Blas (University of Connecticut).

Adult rat brain membrane immunoblot.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-137

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABA(A)R, Beta1

Scientific Background

Gamma-aminobutyric acid receptor subunit beta-1 or -GABA AR-Beta1 is encoded by the gene GABRB1 GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 5 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR-Beta1 is primarily expressed in brain at the cell junction, synapse,and the postsynaptic cell membrane. Diseases associated with GABRB1 include early infantile epileptic encephalopathy 43, neurotic disorder, schizophrenia and autism.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N96/55

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Gabrb1

Molecular Weight

55 kDa

Immunogen

Fusion protein amino acids 327-450 of mouse GABA-A-R-Beta1 (accession number P50571) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2109406

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.64

Dilution Ranges

WB: 1:1000
IHC: 1:200
ICC: 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity against GABA-A-R-Beta2 or -Beta3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

AW061132; Gamma-aminobutyric acid receptor subunit beta-1; B230208N19Rik; GABA(A receptor subunit beta-1; GABA(A receptor beta 1; GABA(A receptor subunit beta-1; GABA-A receptor beta-1 polypeptide; Gabrb-1; GABRB1; Gamma aminobutyric acid (GABA A receptor beta 1; Gamma Aminobutyric Acid A Receptor Beta 1; Gamma Aminobutyric Acid Receptor beta-1; Gamma-aminobutyric acid (GABA A receptor subunit beta 1; Gamma-aminobutyric acid receptor subunit beta-1; GARB1; GBRB1_HUMAN

UniProt ID

P50571
UniProt Summary: Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. Beta-containing GABAARs can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness.

NCBI Gene ID

14400

Shipping

Shipped on ice packs

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Anti-GABA-A-R, Beta1 Antibody (N96/55)

SKU: 75-137

Product Details

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