Anti-HCN1 Antibody (N70/28)

Name: Anti-HCN1 Antibody
Brand: NeuroMab
SKU: 75-110
Price: 394.0 USD
Availability: InStock

Our mouse HCN1 monoclonal antibody from NeuroMab is produced in-house from clone N70/28. It detects chicken, gerbil, human, non-human primate, mouse, and rat HCN1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA, EM.

Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel).

Adult rat cerebellum immunohistochemistry.

Adult rat hippocampus immunohistochemistry.

Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel).

Click on image to zoom

Species Reactivity

Chicken, Gerbil, Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, EM, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

Trial Size

SKU: 75-110

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

HCN1

Scientific Background

HCN1 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN1 is found in the hippocampus, neocortex, and cerebellar cortex of brain. Alterations in this protein have been implicated in the disease early infantile epileptic encephalopathy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N70/28

Isotype

IgG1

Applications

ELISA, EM, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Hcn1

Molecular Weight

100 kDa

Immunogen

Fusion protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1 (accession number Q9JKB0); Mouse: 96% identity (128/133 amino acids identical) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Chicken, Gerbil, Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2115181

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.38

Dilution Ranges

WB: 1:500
IHC: 1:500
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity against HCN2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

UniProt ID

Q9JKB0

Shipping

Shipped on ice packs

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Anti-HCN1 Antibody (N70/28)

SKU: 75-110

Product Details

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