Anti-HCN4 Antibody (N114/10)

Name: Anti-HCN4 Antibody
Brand: NeuroMab
SKU: 75-150
Price: 337.0 USD
Availability: InStock

Our mouse HCN4 monoclonal antibody from NeuroMab is produced in-house from clone N114/10. It detects gerbil, human, mouse, pig, and rat HCN4 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat retina membrane immunoblot (left) and immunofluorescence (right). Green = N114/10 staining of the inner nuclear, inner plexiform, ganglion cell and optic fiber layers (INL, IPL, GCL, and OFL), where layers are viewed by differential interference contrast microscopy. Data courtesy of Gloria Partida, Tyler Stradleigh, and Andrew Ishida (UCDavis).

Adult rat thalamus immunohistochemistry.

Western blot analysis of rat SANPC mitochondria showing total and mitochondrial protein expression of HCN4. Image from publication CC-BY-4.0. PMID: 35840807

$Immunoblots of membrane-enriched fraction from adult (Ad) and 9 (P9) or 11 (P11) postnatal days Wt and Sarm1−/− mice probed with anti-HCN4 (Cat. 75-150, 1ug/ml). Image from publication CC-BY-4.0. PMID:38891076$

Adult rat retina membrane immunoblot (left) and immunofluorescence (right). Green = N114/10 staining of the inner nuclear, inner plexiform, ganglion cell and optic fiber layers (INL, IPL, GCL, and OFL), where layers are viewed by differential interference contrast microscopy. Data courtesy of Gloria Partida, Tyler Stradleigh, and Andrew Ishida (UCDavis).

Click on image to zoom

Species Reactivity

Gerbil, Human, Mouse, Pig, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-150

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

HCN4

Scientific Background

HCN4 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN4 is found in the cell membrane as a multi pass membrane protein in the sino-atrial node of the heart. This protein is highly expressed in neurons in the thalamus and other brain regions and testis. Mutations in HCN4 have been found in several types of sinus bradycardia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N114/10

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

HCN4

Molecular Weight

130 kDa

Immunogen

Fusion protein amino acids 1019-1108 (cytoplasmic C-terminus) of human HCN4 (accession number Q9JKA7) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Gerbil, Human, Mouse, Pig, Rat

Antibody Registry ID

AB_2248534

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.77

Dilution Ranges

WB: 1:500
IHC: 1:200
ICC: 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity against other HCN’s

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

UniProt ID

Q9Y3Q4

Shipping

Shipped on ice packs

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Anti-HCN4 Antibody (N114/10)

SKU: 75-150

Product Details

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