Anti-HCN3 Antibody (N141/28)

Name: Anti-HCN3 Antibody
Brand: NeuroMab
SKU: 75-175
Price: 337.0 USD
Availability: InStock

Our mouse HCN3 monoclonal antibody from NeuroMab is produced in-house from clone N141/28. It detects mouse and rat HCN3 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Immunoblot versus lysates of total brain from HCN3 knockout (KO) and wild-type (WT) mice probed with N141/28. Data courtesy of Martin Biel (Ludwig-Maximilians Universität München) and reproduced from the Journal of Biological Chemistry (2013 Cao-Ehlker et al, PMID 23382386).

Adult rat hippocampus immunohistochemistry.

Immunoblot versus lysates of total brain from HCN3 knockout (KO) and wild-type (WT) mice probed with N141/28. Data courtesy of Martin Biel (Ludwig-Maximilians Universität München) and reproduced from the Journal of Biological Chemistry (2013 Cao-Ehlker et al, PMID 23382386).

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Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-175

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

HCN3

Scientific Background

HCN3 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations HCN3 is found in brain, heart, skeletal muscle and lung.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N141/28

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Hcn3 Hac3

Molecular Weight

90 kDa

Immunogen

Fusion protein amino acids 660-779 (cytoplasmic C-terminus) of mouse HCN3 (accession number O88705) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2120025

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.103

Dilution Ranges

WB: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity against other HCN’s

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (Hyperpolarization-activated cation channel 3) (HAC-3)

UniProt ID

O88705

Shipping

Shipped on ice packs

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Anti-HCN3 Antibody (N141/28)

SKU: 75-175

Product Details

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