Anti-HCN2 Antibody (N71/37)

Name: Anti-HCN2 Antibody
Brand: NeuroMab
SKU: 75-111
Price: 394.0 USD
Availability: InStock

Our mouse HCN2 monoclonal antibody from NeuroMab is produced in-house from clone N71/37. It detects human, mouse, and rat HCN2 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and of COS-1 cells transfected with GFP- HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N71/37 (left), K89/34 (middle) or a control (right) TC supe.

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].

Adult rat cerebellum immunohistochemistry.

Adult rat hippocampus immunohistochemistry.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and of COS-1 cells transfected with GFP- HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N71/37 (left), K89/34 (middle) or a control (right) TC supe.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-111

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

HCN2

Scientific Background

HCN2 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN2 is expressed throughout the brain and in high levels in thalamic and brainstem nuclei.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N71/37

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Hcn2

Molecular Weight

95 kDa

Immunogen

Fusion protein amino acids 761-863 (cytoplasmic C-terminus) of rat HCN2 (accession number Q9JKA9) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2279449

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.39

Dilution Ranges

WB: 1:500
IHC: 1:500
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity against HCN1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

UniProt ID

Q9JKA9

Shipping

Shipped on ice packs

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Anti-HCN2 Antibody (N71/37)

SKU: 75-111

Product Details

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