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Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/12 (left) or N52A/42 (right) TC supe.  Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/22 (left) or N52A/42 (right) TC supe.  Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274)..
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged VAPA, VAPB or untagged Kv2.1 plasmid probed with N479/107 (left) or N86/8 (right) TC supe..Representative images of transfected neurons (white arrow) expressing GCaMP or MitoPAGFP (green) and VAP CRISPR-Cas9 sgRNAs (e) and immunostained for VAP (cat. 75-496, 1:100;  white) show reduced VAP immunofluorescence compared to untransfected, Control neurons (white arrowheads), identified using Map2. Similar results were obtained with VAP shRNA KD.  Image from publication CC-BY-4.0. PMID:38177103

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