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Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7 & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8 & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat1 in human A431 cells. The cells were labeled with mouse monoclonal Stat1 (SM2491) antibody (Right), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. Corresponding phase image is shown to the left.
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 ng/ml for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
Immunofluorescence staining of adult hippocampus (sp = stratum pyrimidale, sl = stratum lucidum) from Stonin-2 wild-type (WT) and knockout (KO) mice with N346/9 (red) and K89/34 (green). Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Stonin-2 KO mouse brains probed with N346/9 (left), K89/34 (middle) and N52A/42 (right) TC supes. Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).
Anti-Superoxide dismutase 1 (SOD1) Antibody
Anti-Superoxide dismutase 1 (SOD1) Antibody
Anti-Superoxide dismutase 1 (SOD1) AntibodyAnti-Superoxide dismutase 1 (SOD1) Antibody
Immunoblot versus extracts of heart tissue from WT and SUR2 KO mice probed with N323A/31 TC supe. Data courtesy of William Coetzee and Margaret Rice, New York University SOM.

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