Anti-Pan-SAPAP Antibody (N127/31)

Name: Anti-Pan-SAPAP Antibody
Brand: NeuroMab
SKU: 75-156
Price: 337.0 USD
Availability: InStock

Our mouse Pan-SAPAP monoclonal antibody from NeuroMab is produced in-house from clone N127/31. It detects human, mouse, and rat Pan-SAPAP and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunolabeling of mouse hippocampus identifying GKAP (Anti-SAPAP, Cat no 75-156, green). Nuclear staining done with DAPI. Image kindly provided by Huaye Zhang, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School.

Adult RBM and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Flag-tagged SAPAP1, SAPAP4 or untagged Kv2.1 plasmids and probed with N127/31 TC supe.

Cultured rat interneuron immunofluorescence staining. N127/31 (Pan-SAPAP) = green, rabbit anti-PSD-95 = red. Image courtesy of Dieter Edbauer and Morgan Sheng, Picower Institute.

Adult rat hippocampus immunohistochemistry.

Adult rat brain membrane (RBM) immunoblot.

Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)].

Cultured rat hippocampal pyramidal cell immunofluorescence staining. N127/31 (Pan-SAPAP) = green, rabbit anti-PSD-95 = red.

Immunolabeling of mouse hippocampus identifying GKAP (Anti-SAPAP, Cat no 75-156, green). Nuclear staining done with DAPI. Image kindly provided by Huaye Zhang, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-156

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Pan-SAPAP

Scientific Background

SAPAP1 (also known as disks large associated protein 1), SAPAP2 (also known as disks large associated protein 2), SAPAP3 (also known as disks large associated protein 3) and SAPAP4 (also known as disks large associated protein 4) proteins are members of the SAPAP or DLGAP family of proteins. SAPAP proteins function as scaffolding proteins at excitatory synapses. SAPAP proteins interact with PSD95 family and Shank family proteins to anchor/target neurotransmitter receptors and signaling molecules to the postsynaptic membrane of excitatory synapses.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N127/31

Isotype

IgG2b

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

DLGAP1 DAP1 GKAP DLGAP2 C8orf68 DAP2 ERICH1-AS1 DLGAP3 DAP3 DLGAP4 DAP4 KIAA0964 SAPAP4

Molecular Weight

120 kDa (SAPAP2), 110 kDa (SAPAP1/3/4)

Immunogen

Fusion protein amino acids 772-992 (C-terminus) of rat SAPAP1 (accession number P97836) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10671947

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.82

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Cross-reacts with other SAPAPs

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Disks large-associated protein 1 (SAPAP1) SAPAP-1 Disks large-associated protein 2 (SAPAP2) SAPAP-2 Disks large-associated protein 3 (SAPAP3) SAPAP-3 Disks large-associated protein 4 (SAPAP-4) SAPAP4

UniProt ID

P97836

Shipping

Shipped on ice packs

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Anti-Pan-SAPAP Antibody (N127/31)

SKU: 75-156

Product Details

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