Anti-Pan-Shank Antibody (N23B/49)

Name: Anti-Pan-Shank Antibody
Brand: NeuroMab
SKU: 75-089
Price: 337.0 USD
Availability: InStock

Our mouse Pan-Shank monoclonal antibody from NeuroMab is produced in-house from clone N23B/49. It detects human, mouse, and rat Pan-Shank and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Cultured rat hippocampal neuron immunofluorescence: N23B/49 (Pan-Shank) = red, rabbit anti-PSD-95 = green, merge = yellow (bottom right). Image courtesy of Dr. Albert Hung, Picower Institute, MIT.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/49 TC supe or a lane loading control.

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].

Immature synaptic structures in 11 DIV hippocampal neurons. Immunofluorescence images of 11 DIV HC following treatment with 100 μM glutamate for 15 min showing specific labelling of Shank (Cat. 75-089, 1:100). Image from publication CC-BY-4.0. PMID:38660466

Cultured rat hippocampal neuron immunofluorescence: N23B/49 (Pan-Shank) = red, rabbit anti-PSD-95 = green, merge = yellow (bottom right). Image courtesy of Dr. Albert Hung, Picower Institute, MIT.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-089

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Pan-Shank

Scientific Background

This antibody is a pan antibody that recognizes SH3 and multiple ankyrin repeat domains protein 1 (Shank 1), SH3 and multiple ankyrin repeat domains protein 2 (Shank 2) and SH3 and multiple ankyrin repeat domains protein 3 (Shank3). Shank proteins act as a scaffold protein anchoring and connecting membrane bound receptors and other proteins to the cytoskeleton in neurons to enable cell signaling.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N23B/49

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Shank1 Shank2 Cortbp1 Kiaa1022 PROSAP1 Shank3 Kiaa1650 Prosap2 PSAP2

Molecular Weight

160 kDa

Immunogen

Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (accession number Q9QX74) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10672418

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.19

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Recognizes Shank1, Shank2 and Shank3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

SH3 and multiple ankyrin repeat domains protein 1 (Shank1) (GKAP/SAPAP-interacting protein) (SPANK-1) (Somatostatin receptor-interacting protein) (SSTR-interacting protein) (SSTRIP) (Synamon) SH3 and multiple ankyrin repeat domains protein 2 (Shank2) (Cortactin-binding protein 1) (CortBP1) (GKAP/SAPAP-interacting protein) (Proline-rich synapse-associated protein 1) (ProSAP1) (SPANK-3) SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

UniProt ID

Q9QX74

Shipping

Shipped on ice packs

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Anti-Pan-Shank Antibody (N23B/49)

SKU: 75-089

Product Details

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