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Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).Immunocytochemical labeling of nSMase2 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-nSMase2 (SP4061) antibody in the absence (Left) or presence (Right) of blocking peptide (SX4065). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.
Western blot of nSMase3a and nSMase3b GFP transfectants (A) and nSMase3 siRNA knockdown in C2C12 myotubes (B). Both blots were probed with anti-nSMase3 (C-terminal region) rabbit polyclonal antibody (SP0281). (Image provided by Dr. Jennifer Moylan, Dept. Physiology, University of Kentucky).Immunocytochemical labeling of nSMase3 in aldehyde-fixed and NP-40-permeabilized mouse C2C12 cells. The cells were labeled with rabbit polyclonal anti-nSMase3 (SP0281) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Immunohistochemistry of adult rat brain hippocampus showing specific staining of Nurr1. Tissue was fixed in paraformaldehyde and cut into 20 micron sections.Western Blot of 20 ug of human hippocampus tissue lysate (lanes 2 and 4) showing specific immunolabeling of Nurr1. Peptide absorption control lane 3.
Anti-Obestatin Antibody
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged Kv2.1, GFP-tagged OCRL or RFP-tagged INPP5b plasmids and probed with N166A/26 (left) and another cross-reacting mouse OCRL antibody (right).
Anti-OCRL/INPP5b Antibody (BSA and Azide free)
Anti-OCRL/INPP5b Antibody Biotin Conjugate
Anti-OCRL/INPP5b Antibody FL490 Conjugate
Anti-OCRL/INPP5b Antibody FL550 Conjugate
Anti-OCRL/INPP5b Antibody FL594 Conjugate
Anti-OCRL/INPP5b Antibody FL650 Conjugate
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.
NeuroMab Anti-Olig1 Antibody
Sale priceFrom $142
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