Anti-nSMase2 (C-terminal region) Antibody

Name: Anti-nSMase2 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SP4061
Price: 348.0 USD
Availability: InStock

Our nSMase2 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat nSMase2 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).

Immunocytochemical labeling of nSMase2 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-nSMase2 (SP4061) antibody in the absence (Left) or presence (Right) of blocking peptide (SX4065). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.

Representative immunoblots showing expression of nSMase2 (Cat. SP4061, 1:1000) at the indicated timepoints (0 to 48 h) following HIVRF infection of H9 cells. Image from publication CC-BY-4.0. PMID: 37406092

Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: SP4061

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

nSMase2 (C-terminal region)

Scientific Background

Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependance. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase2 (also known as SMPD3) is expressed primarily in brain, and co-localizes with Golgi markers in several cell lines. The activity and phosphorylation of nSMase2 is upregulated in response to oxidative stress. In addition, nSMase2 binds calcineurin, and this interaction inhibits nSMase2 activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

SMPD3

Molecular Weight

Immunogen

nSMase2 synthetic peptide corresponds to amino acids in the C-terminal region of human nSMase2. This sequence is highly conserved in rat and mouse nSMase2, and has low homology to other nSMase family members.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 78 kDa* band corresponding to nSMase2 on SDS-PAGE immunoblots of adult mouse brain and rat PC12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

SMPD3,neutral sphingomyelinase 2, nSMase sphingomyelin

UniProt ID

Q9NY59

Shipping

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Anti-nSMase2 (C-terminal region) Antibody

SKU: SP4061

Product Details

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