Anti-Nuclei Recombinant Chicken Chimeric mAb (235-1)

Our chicken Anti-Nuclei recombinant monoclonal antibody is a chimeric antibody derived from clone 235-1. It detects human Nuclei and is purified by affinity chromatography. Works in ELISA, IHC, ICC.

Immunofluorescent staining for detection of nuclei in HeLa cells using Aves Labs anti-Nuclei (human-specific) chicken recombinant monoclonal antibody (Cat No. 78-611). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-Nuclei antibody (Cat No. 78-611) was incubated with the sample at a dilution of 1:1000 (1 ug/mL) at 4C overnight. After washing with PBST, the cells were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. The staining revealed a complete overlap between the signal from the chicken anti-Nuclei antibody and DAPI nuclear stain.
Immunofluorescent staining of HeLa and NIH3T3 cells using Aves Labs anti-Nuclei (human specific) chicken recombinant antibody [235-1](Cat No. 78-611). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-Nuclei antibody (Cat No. 78-611) was incubated with the sample at a dilution of 1:100 (10 ug/mL) at 4C overnight. After washing with PBST, the cells were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. The staining revealed a complete overlap between the signal from the chicken anti-Nuclei antibody and DAPI nuclear stain in HeLa cells, with no staining observed in mouse NIH3T3 cells.
Immunofluorescent staining for detection of nuclei in HeLa cells using Aves Labs anti-Nuclei (human-specific) chicken recombinant monoclonal antibody (Cat No. 78-611). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-Nuclei antibody (Cat No. 78-611) was incubated with the sample at a dilution of 1:1000 (1 ug/mL) at 4C overnight. After washing with PBST, the cells were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. The staining revealed a complete overlap between the signal from the chicken anti-Nuclei antibody and DAPI nuclear stain.
Immunofluorescent staining for detection of nuclei in HeLa cells using Aves Labs anti-Nuclei (human-specific) chicken recombinant monoclonal antibody (Cat No. 78-611). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-Nuclei antibody (Cat No. 78-611) was incubated with the sample at a dilution of 1:1000 (1 ug/mL) at 4C overnight. After washing with PBST, the cells were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. The staining revealed a complete overlap between the signal from the chicken anti-Nuclei antibody and DAPI nuclear stain.
Immunofluorescent staining for detection of nuclei in HeLa cells using Aves Labs anti-Nuclei (human-specific) chicken recombinant monoclonal antibody (Cat No. 78-611). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-Nuclei antibody (Cat No. 78-611) was incubated with the sample at a dilution of 1:1000 (1 ug/mL) at 4C overnight. After washing with PBST, the cells were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. The staining revealed a complete overlap between the signal from the chicken anti-Nuclei antibody and DAPI nuclear stain.
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Human
ELISA, ICC, IHC
Chicken
490
550
594
650
Biotin
Carrier-Free

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SKU: 78-611

Volume: 100 µL
Price:
$481.00
Ships: 1-2 business days

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