Anti-Nuclei Recombinant Chicken Chimeric mAb (BSA and Azide free) (235-1)

Name: Anti-Nuclei Recombinant Chicken Chimeric mAb (BSA and Azide free)
Brand: Aves Labs
SKU: 78-611-CF
Price: 522.0 USD
Availability: InStock

Our chicken Anti-Nuclei carrier-free recombinant monoclonal antibody is a chimeric antibody derived from clone 235-1. It detects human Nuclei and is purified by affinity chromatography. Works in ELISA, IHC, ICC.

Anti-Nuclei Recombinant Chicken Chimeric mAb (BSA and Azide free)

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Species Reactivity

Human

Applications

ELISA, ICC, IHC

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 78-611-CF

Volume: 100 µL

Variant

Price:

$522.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Nuclei

Scientific Background

This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.6-1.0 mg/mL

Clonality

Recombinant

Clone

235-1

Isotype

IgY

Applications

ELISA, ICC, IHC

Host Species

Chicken

Gene Name

Marker for human cells in xenographic research

Excitation / Emission

Unconjugated

Immunogen

Human cell homogenate.

Immunogen Species

Human

Species Reactivity

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 235-1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone 235-1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

PBS

Dilution Ranges

IHC: 1:100-1:500
ICC: 1:100-1:1000

Conjugate

Unconjugated, Carrier Free

Specificity

Does not cross-react with Arl13a (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by immunocytochemistry on HeLa cells and confirmed to colocalize with DAPI.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

HNA antibody, Human Nuclear Antigen antibody, HuNu antibody

Shipping

Shipped on ice packs

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Anti-Nuclei Recombinant Chicken Chimeric mAb (BSA and Azide free) (235-1)

SKU: 78-611-CF

Product Details

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