Anti-Olig1 Antibody (N149/25)

Name: Anti-Olig1 Antibody
Brand: NeuroMab
SKU: 75-180
Price: 337.0 USD
Availability: InStock

Our mouse Olig1 monoclonal antibody from NeuroMab is produced in-house from clone N149/25. It detects mouse and rat Olig1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.

Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-180

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Olig1

Scientific Background

Oligodendrocyte transcription factor 1, Basic Domain, Helix-Loop-Helix Protein, Class B, 6 or Class E Basic Helix-Loop-Helix Protein 21 is encoded by the gene Olig1. Olig1 is a transcription factor and a member of the basic helix-loop-helix (bHLH) factor family. Olig1 promotes formation and maturation of oligodendrocytes (oligodendrogensis). Olig1 is expressed in spinal cord and brain during development, and by oligodendrocytes after late embryonic development. Olig1 is a useful marker for Oligodendrocyte staining. Diseases associated with this gene include Oligodendroglioma and Grade Iii Astrocytoma.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N149/25

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Olig1

Molecular Weight

30 kDa

Immunogen

Fusion protein amino acids 1-67 (N-terminus) of mouse Olig1 (accession number Q9JKN5) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2157532

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.108

Conjugate

Unconjugated

Specificity

No cross-reactivity against Olig2

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Oligodendrocyte transcription factor 1 (Oligo1) (Oligodendrocyte-specific bHLH transcription factor 1)

UniProt ID

Q9JKN5

Shipping

Shipped on ice packs

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Anti-Olig1 Antibody (N149/25)

SKU: 75-180

Product Details

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