Anti-Nuclei Recombinant Chicken Chimeric mAb BTN Conjugate (235-1)

Name: Anti-Nuclei Recombinant Chicken Chimeric mAb BTN Conjugate
Brand: Aves Labs
SKU: 78-611-BTN
Price: 562.0 USD
Availability: InStock

Our chicken Nuclei biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone 235-1. It detects human Nuclei and is purified by affinity chromatography. Works in ELISA, IHC, ICC, WB.

Anti-Nuclei Recombinant Chicken Chimeric mAb BTN Conjugate

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Species Reactivity

Human

Applications

ELISA, ICC, IHC

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-611-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Nuclei

Scientific Background

This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

235-1

Isotype

IgY

Applications

ELISA, ICC, IHC

Host Species

Chicken

Gene Name

Marker for human cells in xenographic research

Immunogen

Human cell homogenate.

Immunogen Species

Human

Species Reactivity

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 235-1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone 235-1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

Biotin

Specificity

Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

HNA antibody, Human Nuclear Antigen antibody, HuNu antibody

Shipping

Shipped on ice packs

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Anti-Nuclei Recombinant Chicken Chimeric mAb BTN Conjugate (235-1)

SKU: 78-611-BTN

Product Details

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