SfN 2024 Free Antibody Promotion

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Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser234. This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A: Mut) expressed in HeLa cell lysate.
PhosphoSolutions Anti-Beclin1 (Ser234) Antibody
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Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser295. This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A: Mut) expressed in HeLa cell lysate.
PhosphoSolutions Anti-Beclin1 (Ser295) Antibody
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Immunofluorescence of transfected COS-7 cells specifically labeling positive beta Catenin cells using our anti-beta Catenin antibody (1:200, red) and anti-FLAG antibody (green). The yellow color results from the overlap of cells labeled with both antibodies. DNA is labeled with DAPI (blue).Western blot of rat whole brain lysate showing specific immunolabeling of the ~92 kDa beta Catenin protein.
PhosphoSolutions Anti-beta Catenin Antibody
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Western blot of 293 cells mock transfected (lane 1) or transiently transfected with pLenti6/TR lentiviral vector (lanes 2 & 3). Blots were probed with anti-Bsd (lanes 1-3). Molecular weight (MW) standards show that Bsd is expressed as a 13 kDa band in only the transfected cells.Immunocytochemical labeling of Blasticidin S Deaminase (Bsd) in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were labeled with anti-Bsd (BP1231) and detected using appropriate secondary antibody conjugated to Texas Red. (Images provided by Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Center).
PhosphoSolutions Anti-Blasticidin S Deaminase Antibody
$348
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).
PhosphoSolutions Anti-c-Abl (Tyr-245), Phosphospecific Antibody
$348
Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).Representative ABL1p-Y412 (Cat. AP1271) immunohistochemistry of tumors or para-carcinom in human breat cancer tissue are shown. Image from publication CC-BY-4.0. PMID: 39060421
PhosphoSolutions Anti-c-Abl (Tyr-412), Phosphospecific Antibody
$348
Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-c-Cbl (CM1591; lanes 1 & 2) or anti-c-Cbl (Tyr-700) (CM1611; lanes 3 & 4).
PhosphoSolutions Anti-c-Cbl (Tyr-700), Phosphospecific Antibody
$348
Western blot of GST fusion protein containing human C-Raf. The blot was probed with polyclonal anti-C-Raf (C-terminus) antibody in the presence (lanes 2-4) or absence (lane 1) of C-Raf (C-terminus) blocking peptide (lane 2), C-Raf (Ser-471) peptide (lane 3), or unrelated peptide (lane 4).
PhosphoSolutions Anti-C-Raf (C-terminus) Antibody
$292
Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-C-Raf (N-terminal region) (lanes 1 & 2) or anti-C-Raf (Ser-471) (lanes 3 & 4).
PhosphoSolutions Anti-C-Raf (S471) [B-Raf (S579)/A-Raf (S432)], Phosphospecific Antibody
$348
Western blot analysis of mouse SYF cells transformed with c-Src then left untreated (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-c-Src (Tyr-215) (lanes 1 & 2), anti-c-Src (N-terminal region) (lanes 3 & 4), and anti-c-Src (Tyr-530) (lanes 5 & 6).
PhosphoSolutions Anti-c-Src (Tyr-215) [conserved site], Phosphospecific Antibody
$348
Western blot analysis of mouse SYF cells transformed with c-Src then left untreated (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-c-Src (Tyr-215) (lanes 1 & 2), anti-c-Src (N-terminal region) (lanes 3 & 4), and anti-c-Src (Tyr-530) (lanes 5 & 6).
PhosphoSolutions Anti-c-Src (Tyr-530) [conserved site], Phosphospecific Antibody
$348
Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained and photo was provided courtesy of QBMCellScience.Anti-Calcitonin Gene Related Peptide (CGRP) Antibody
PhosphoSolutions Anti-Calcitonin Gene Related Peptide (CGRP) Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes). Astrocytes (1 × 106 cells/ml) were cultured for 24 h without treatment or with METH (10 μM), clade B gp120 (50 ng/ ml), or clade C gp120 (50 ng/ ml) alone or METH in combination with clade B or C gp120. After incubation, the cells were fixed, permeabilized, and stained for (A) DRD-2, (B) phospho-CaMK II (Cat. p1005-286) (C) phospho-CaMK IV (green) or GFPA (red) and DAPI (blue). The cells were imaged using a confocal microscope at 630x and analyzed using immunocytochemistry. Image from publication CC-BY-4.0.
PhosphoSolutions Anti-CaM Kinase II (Thr286) Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr306 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 minutes).
PhosphoSolutions Anti-CaM Kinase II (Thr306) Antibody
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Western blot of lysed HFF cells that were serum starved for 24 hours and then refed for 30 minutes showing specific immunolabeling of the ~130-260 kDa CaSR protein.
PhosphoSolutions Anti-CaSR Antibody
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Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).Immunocytochemical labeling of caveolin-1 phosphorylation in rabbit spleen fibroblasts. The cells were treated with pervanadate (1 mM) for 30 min, then fixed with paraformaldehyde and labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 (Tyr-14) antibodies. The antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Caveolin-1 (Tyr-14), Phosphospecific Antibody
$379
Western blot of lysates from BEAS normal lung cells (lane 1), NCIH28 mesothelial cells (lane 2), NCI-H1299 lung cancer cells (lane 3), LNCaP prostate cancer cells (lane 4), MCF7 breast cancer cells (lane 5), and A431 skin adenocarcinoma (lane 6). The blot was probed with mouse monoclonal anti-CD147 (CM0361) at 1:1000.Immunocytochemical labeling of CD147 in paraformaldehyde fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD147 (CM0361). The antibody was detected using goat anti-mouse Ig:DyLight® 594.
PhosphoSolutions Anti-CD147/Emmprin/Basigin (Extracellular region) Antibody
$379
Western blot of lysates from SK-MES-1 squamous carcinoma (lane 1), MDA-MB-231 breast carcinoma (lane 2), MeWo melanoma (lane 3), A431 skin adenocarcinoma (lane 4), LNCaP prostate cancer cells (lane 5), MCF7 breast cancer cells (lane 6) and NCI-H1915 lung cancer cells. The blot was probed with mouse monoclonal anti-CD147 (CM0521) at 1:1000.
PhosphoSolutions Anti-CD147/Emmprin/Basigin (Extracellular region) Antibody
$348
Western blot of human A549 lung carcinoma (lane 1), NCI-H446 small cell lung carcinoma (lane 2), NCI-H1299 lung carcinoma (lane 3), MDA-MB-231 breast carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-CD155 (CM0481) at 1:500.Immunocytochemical labeling of CD155 in aldehyde fixed and NP-40 permeabilized human NCI-H446 small cell lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD155 (CM0481). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD155/PVR (Extracellular region) Antibody
$379
Western blot of native (lane 1 and 3) and denatured (lane 2 and 4) lysates of human THP-1 monocytes. The blot was probed with mouse monoclonals anti-CD244/2B4/SLAMF4 (CM0531) (lanes 1 and 2) or anti-CD244/2B4/SLAMF4 (CM0851). Both antibodies were used at 1:250.Immunocytochemical labeling of CD244 in aldehyde fixed and NP-40 permeabilized PMA-differentiated human THP-1 cells. The cells were labeled with mouse monoclonal anti-CD244/2B4/SLAMF4 (CM0531). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody
$379
Western blot of native (lane 1 and 3) and denatured (lane 2 and 4) lysates of human THP-1 monocytes. The blot was probed with mouse monoclonals anti-CD244/2B4/SLAMF4 (CM0531) (lanes 1 and 2) or anti-CD244/2B4/SLAMF4 (CM0851). Both antibodies were used at 1:250.Immunocytochemical labeling of CD244 in aldehyde fixed and NP-40 permeabilized PMA-differentiated human THP-1 cells. The cells were labeled with mouse monoclonal anti-CD244/2B4/SLAMF4 (CM0851). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD244/2B4/SLAMF4 (Extracellular region) M085 Antibody
$379
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M007). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$379
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M010). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$379
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M024). The antibody was detected using goat anti-mouse DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD44 (CM0241) for ELISA capture of human recombinant CD44 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
PhosphoSolutions Anti-CD44 (Extracellular region) Antibody
$379

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