Anti-CD147/Emmprin/Basigin (Extracellular region) Antibody (M036)

Name: Anti-CD147/Emmprin/Basigin (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: CM0361
Price: 379.0 USD
Availability: InStock

Our CD147/Emmprin/Basigin (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD147/Emmprin/Basigin (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Western blot of lysates from BEAS normal lung cells (lane 1), NCIH28 mesothelial cells (lane 2), NCI-H1299 lung cancer cells (lane 3), LNCaP prostate cancer cells (lane 4), MCF7 breast cancer cells (lane 5), and A431 skin adenocarcinoma (lane 6). The blot was probed with mouse monoclonal anti-CD147 (CM0361) at 1:1000.

Immunocytochemical labeling of CD147 in paraformaldehyde fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD147 (CM0361). The antibody was detected using goat anti-mouse Ig:DyLight® 594.

Representative Standard Curve using mouse monoclonal CD147 (CM0361) for ELISA capture of human recombinant CD147
extracellular region with a His tag. Capture was detected using anti-His tag antibody followed by appropriate secondary antibody
HRP conjugate.

Western blot of lysates from BEAS normal lung cells (lane 1), NCIH28 mesothelial cells (lane 2), NCI-H1299 lung cancer cells (lane 3), LNCaP prostate cancer cells (lane 4), MCF7 breast cancer cells (lane 5), and A431 skin adenocarcinoma (lane 6). The blot was probed with mouse monoclonal anti-CD147 (CM0361) at 1:1000.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

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Data Sheet

SKU: CM0361

Volume: 100 µL

Variant

Price:

$379.00

Quantity:

Ships: 1-2 business days

Product Details

Target

CD147/Emmprin/Basigin (Extracellular region)

Scientific Background

D147 (Basigin/EMMPRIN) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. CD147 is a glycosylated protein with four known isoforms with isoform 2 being the most abundantly expressed. In many cancers, CD147 is overexpressed and stimulates the secretion of extracellular matrix metalloproteinases by tumor fibroblasts, which can promote tumor progression. CD147 is co-expressed with the amino acid transporter, CD98h, in metabolically active cells such as lymphocytes, macrophages, and tumor cells. CD147 interaction with caveolin and cyclophilins regulates its functions, and this protein may function outside of tumor cells through cell shedding and microvesicle release.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M036

Isotype

IgG2a

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

Gene Name

CD147

Molecular Weight

38-58

Immunogen

Clone (M036) was generated from a proprietary antigen related to the native human CD147 expressed in NCI-H1915 lung cancer cell line.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:2000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

Clone M036 mouse monoclonal antibody detects a 38-58 kDa* protein on SDS-PAGE immunoblots of human BEAS, NCIH28, NCI-H1299, LNCaP, and A431 cells, as well as human brain tissues. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

5F7; Basigin; EMMPRIN; EMPRIN; TCSF; OK blood group antigen; collagenase stimulatory factor; extracellular matrix metalloproteinase inducer; leukocyte activation antigen M6; tumor cell-derived collagenase stimulatory factor; Collagenase stimulatory factor Extracellular matrix metalloproteinase,EMMPRIN; CD147; BSG

UniProt ID

P35613
UniProt Summary: [Isoform 1]: Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors. May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes.

NCBI Gene ID

682

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Anti-CD147/Emmprin/Basigin (Extracellular region) Antibody (M036)

SKU: CM0361

Product Details

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