Polyclonal Antibodies

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Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Aves Labs Anti-C-MYC Epitope Tag Antibody
$304
Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase (Cat. 2025-THRAB, green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0.1 to 0.3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.
PhosphoSolutions Anti-Tyrosine Hydroxylase Antibody
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Western blot of rat striatal lysate showing specific immunolabeling of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Dopamine Transporter (Thr53) Antibody
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Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2 (Cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
PhosphoSolutions Anti-Microtubule Associated Protein 2 (MAP2) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser845 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Effects of striatal slice treatment with NMDA (25 μM, 5 min), dopamine (DA, 10 μM, 15 min), or both on PKA-dependent phosphorylation of Ser845 GluR1 (top) and Thr34 DARPP-32 (bottom). Image from publication CC-BY-4.0. PMID: 35835216
PhosphoSolutions Anti-GluR1-Subunit (Ser845) Antibody
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Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).Immunohistochemical staining of inhibitory neurons (red; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY, (1:500).
Aves Labs Anti-Glutamic Acid Decarboxylase-67 Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
PhosphoSolutions Anti-NMDA NR2B Subunit (Tyr1472) Antibody
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Western blot of HEK293 lysate showing specific immunolabeling of the ~160 kDa MerTK phosphorylated at Tyr749/753/754 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 60 minutes).Western blot image of human THP-1 cells treated with γ-Carboxylated Gas6 at a 10 nM concentration showing increased MerTK phosphorylation (Cat. p186-749). Image from publication CC-BY-4.0. PMID: 38673989
PhosphoSolutions Anti-MerTK (Tyr749/753/754) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT. Immunostaining of rat brainstem showing specific labeling using our anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.
PhosphoSolutions Anti-ChAT (Choline Acetyltransferase) Antibody
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Immunofluorescent staining of COS-7 cells expressing S100B-flag using Aves Labs anti-S100B antibody (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) shows nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-S100B signal and anti-flag in transfected cells.Sagittal section of formalin fixed, paraffin-embedded rat brain showing staining of S100B. Images at right show higher magnification of indicated areas of interest (cerebellum, hippocampus, and cortex). Sections were stained with Aves Labs anti-S100B antibody at 1:500 dilution and detected with anti-chicken HRP.
Aves Labs Anti-S100B Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71 kDa FMRP protein phosphorylated at Ser499 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
PhosphoSolutions Anti-FMRP (Ser499) Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr306 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 minutes).
PhosphoSolutions Anti-CaM Kinase II (Thr306) Antibody
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Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes). Astrocytes (1 × 106 cells/ml) were cultured for 24 h without treatment or with METH (10 μM), clade B gp120 (50 ng/ ml), or clade C gp120 (50 ng/ ml) alone or METH in combination with clade B or C gp120. After incubation, the cells were fixed, permeabilized, and stained for (A) DRD-2, (B) phospho-CaMK II (Cat. p1005-286) (C) phospho-CaMK IV (green) or GFPA (red) and DAPI (blue). The cells were imaged using a confocal microscope at 630x and analyzed using immunocytochemistry. Image from publication CC-BY-4.0.
PhosphoSolutions Anti-CaM Kinase II (Thr286) Antibody
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Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.
Aves Labs Anti-DYKDDDDK Epitope Tag/FLAG Antibody
$304
Representative images of heterozygous (top) and homozygous (bottom) fibroblasts stained with AURKB (C, prophase) or survivin (metaphase, D) (red; shown in grayscale) and FITC-conjugated CREST (Cat. 15-235-F, 1:100, green; shown in grayscale). Image from publication CC-BY-4.0. PMID:28272472Immunofluorescence staining of fibroblasts shows partial and transient localization of WDR62 (red) in prometaphase kinetochores (CREST, cat. 15-235-F, 1:100). Image from publication CC-BY-4.0. PMID:28272472
Antibodies Incorporated Anti-Centromere Protein Antibody - FITC Labeled
$268
Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody
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Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40.
PhosphoSolutions Anti-Tyrosine Hydroxylase (Ser40) Antibody
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Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).
Aves Labs Anti-Peripherin Antibody
$295
Immunocytochemical staining of mouse brain cortical neurons in culture. NFL immunoreactivity is red and yellow. Blue staining is DAPI nuclear staining. Anti-NFL (1:1000 dilution). Dr. Felix Eckenstein, University of Vermont.Immunohistochemical staining of a paraffin-embedded section through mouse cerebellum. Anti-NFL immunoreactivity (Aves Labs, 1:500 dilution) was detected using HRP-labeled goat anti-chicken IgY (Aves Cat.No. H-1004).
Aves Labs Anti-NFL (Neurofilament Light Chain) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa GABAA β3 protein phosphorylated at Ser408/409 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-GABAA Receptor, ß3 (Ser408/409) Antibody
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Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (Cat.1099-MAP2, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP (Cat.1120-MBP , 1:5000, green). The blue is DAPI revealing nuclear DNA.Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
PhosphoSolutions Anti-Microtubule Associated Protein 2 (MAP2) Antibody
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Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.Mouse auditory neurons expressing a V5-tagged reporter protein were stained with Aves Labs chicken anti-V5 antibody at 1:500. Photo courtesy of Thomas Coate.
Aves Labs Anti-V5 Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (Cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (Cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41–10 μM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853
PhosphoSolutions Anti-GluR1-Subunit (Ser831) Antibody
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Immunostaining of HeLa cells showing specific labeling of Ki-67 (Cat. Ki67-0100, 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.Western blotting of HeLa cell lysate (10 µg/lane) with Ki-67 antibody at 2 µg/mL dilution and detected with anti-chicken HRP.
Aves Labs Anti-Ki-67 Antibody
$295

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