Polyclonal Antibodies

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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA receptor.Immunostaining of rabbit retina showing NR2A in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.
PhosphoSolutions Anti-NMDA NR2A Subunit (100 ul) Antibody
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Mixed neuron-glial cultures stained with CPCA-GAP43 (green) and rabbit antibody to alpha-II spectrin (RPCA-aII-Spec, red), and DNA (blue). The GAP43 antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembraneous cytoskeleton and vesicles. Photo by Dr. Gerry Shaw, Univ. Florida.GAP-43 immunoreactivity in a neuroblast within a neurosphere culture. Chicken anti-GAP-43 antibody (1:500 dilution), Fluorescein-goat anti-chicken IgY secondary antibody (Aves Labs, 1:500). Photomicrograph by Hoda Ilias, Aves Labs.
Aves Labs Anti-Growth-Associated Protein-43 Antibody
$295
A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.
Aves Labs Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody
$470
Aves Labs Goat Anti-Chicken IgY (H&L) Secondary Antibody, HRP
$184
Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3405).
PhosphoSolutions Anti-MuRF1 (C-terminal region) Antibody
$340
Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 30’ showing the specific immunolabeling of the ~39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 (red, 1:250). Photo courtesy of Patsy Ruegg.
PhosphoSolutions Anti-p38 MAPK (Thr180/Tyr182) Antibody
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Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser234. This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A: Mut) expressed in HeLa cell lysate.
PhosphoSolutions Anti-Beclin1 (Ser234) Antibody
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Western blot of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~32 kDa cleaved actin fragment (fractin). Fractin (Cat. 592-FRAC, 1:5000; red) immunostaining of mouse brain signifying the emergence of damaged/dying neurons in entorhinal cortex (EC). Nuclei stained with DAPI (blue). Final image shows fractin+ cells (red) among YFP+ cells (green) at 7 dpi. Image from publication CC-BY-4.0. PMID:36468693
PhosphoSolutions Anti-Fractin Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68/70 kDa ChAT.
PhosphoSolutions Anti-ChAT (Choline Acetyltransferase) Antibody
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Western blot of 10ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.Immunostaining of cultured mouse caudate neurons showing punctate distribution of synapsin (Cat. 1925-SYNP, 1:1000, green) and MAP (red). Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synapsin I Antibody
$445
Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
PhosphoSolutions Anti-NMDA NR2B Subunit Antibody
$445
Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein (Cat. 1120-MBP, 1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.Western blot of rat cortical lysate showing specific immunolabeling of the ~18 kDa and ~22 kDa MBP protein.
PhosphoSolutions Anti-Myelin Basic Protein (MBP) Antibody
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Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 (Cat. 1538-OLIG2, red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~32 kDa Olig2 protein.
PhosphoSolutions Anti-Olig2 Antibody
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Immunofluorescent staining of HeLa cells using 2 µg/mL Aves Labs chicken anti-Calnexin (CANX) (Cat. CANX-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). Anti-Calnexin specifically stains the Endoplasmic Reticulum in HeLa cells. ​Western blotting of various cell lysates with Aves Labs chicken anti-Calnexin (CANX) antibody (0.05 µg/ml) and detected with anti-chicken HRP. Aves Labs Chicken anti-Calnexin recognizes endogenous Calnexin in all the cell lysates at ~90 kDa.
Aves Labs Anti-Calnexin Antibody
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β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (Cat. ET-HA) fused to their N-termini. Actin (Cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897
Aves Labs Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody
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Immunocytochemical staining of NSE (green, 1:1000 dilution) and Glial Fibrillary Acidic Protein (GFAP, rabbit, 1:1000 dilution). Photomicrograph by Dr. Gerry Shaw (EnCor Biotechnology).Western blot of adult mouse brain homogenate showing a single band at the correct MW (47 kDa), dilution 1:100000.
Aves Labs Anti-Neuron-Specific Enolase Antibody
$470
Beta-Amyloid-positive “neuritic plaque” (shown in higher magnification) in cerebral cortex as seen in a post-mortum specimen taken from an Alzheimer’s disease patient. Picture courtesy of Dr. Randy Woltjer, Oregon Health & Sciences University.Immunohistochemistry of a capillary showing beta-amyloid peptide in the wall of the vessel. Paraffin-embedded section of a human brain from a patient with diagnosed Alzheimer's Disease. Primary antibody -- 1:500; secondary antibody -- 1:1000 HRP-Goat anti-chicken IgY (Aves Labs). Dr. Randy Woltjer, Dept Pathology, OHSU
Aves Labs Anti-Beta-Amyloid Peptide (N-terminus) Antibody
$470
Immunofluoresence of COS7 cells expressing mScarlet3 using chicken anti-mScarlet3 antibody (green) and showing mScarlet3 autofluorescence (red). The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from chicken anti-mScarlet3 antibody and mScarlet3 autofluoresence in transfected cells.Western blotting of mock or mScarlet3 transfected COS-7 cell lysates with chicken anti-mScarlet3 antibody (0.5 ug/ml) and detected with anti-chicken HRP. Chicken anti-mScarlet3 recognizes exogenous mScarlet3 in COS-7 cells.​
Aves Labs Anti-mScarlet3 Antibody
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Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8).Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.
PhosphoSolutions Anti-Myosin Light Chain (Ser-19), Phosphospecific Antibody
$340
Immunofluorescence of mouse lung showing specific labeling of alveolar type 2 epithelial cells using anti-Prosurfactant Protein C antibody in red. Nuclei are stained blue.
PhosphoSolutions Anti-Prosurfactant Protein C Antibody
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Western blot of connexin35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin-35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.
PhosphoSolutions Anti-Connexin 35/36 (Ser276) Antibody
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Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser23/24 in the second lane (-). Phosphospecificity is shown in the first lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).Western blots showing specific labeling of S23/S24 TnI, cat. p2010-2324 (upper) and TnI; cat. 2010-TnI (lower) in protein lysates of mouse cardiomyocytes. Image from publication CC-BY-4.0. PMID: 36424917
PhosphoSolutions Anti-Troponin I (Cardiac) Ser23/24 Antibody
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Western blot of rat mid brain membrane lysate showing specific immunolabeling of the ~76 kDa SERT protein phosphorylated at Thr276 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Serotonin Transporter (Thr276) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser549 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes). Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser549 (Cat. p1560-549, green, 1:500). Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synapsin (Ser549) Antibody
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