Anti-Centromere Protein Antibody

Name: Anti-Centromere Protein Antibody
Brand: Antibodies Incorporated
SKU: 15-235
Price: 179.0 USD
Availability: InStock

Our Anti-Centromere proteins human polyclonal primary antibody from Antibodies Incorporated is produced in-house. It detects Centromere proteins, and is purified by Protein A chromatography. It is great for use in ICC.

Immunolabeling of spreaded WT mouse pachytene spermatocytes positively labeling CREST/Centromere Protein (red, Cat 15-235, 1:20) and SYCP3 (green). Image kindly provided by Fernando Sánchez Sáez and Alberto M. Penás from Universidad de Salamanca, Spain.

Immunostaining of Vipera berus (Reptile, snake) synaptonemal complexes identifying the lateral elements of meiotic bivalents (Anti-SYCP3, green), the centromere proteins (Anti-ACA, Cat 15-235, 1:250, red), and nuclei staining with DAPI (blue). Image kindly provided by Victor Spangenberg, Vavilov Institute of General Genetics, Moscow, Russia.

Immunofluorescence images of two siTTLL11 anaphase cells showing chromosome segregation defects including a lagging chromosome (white arrows). Kinetochores were labeled with CREST (red) and DNA with DAPI (blue). Image from publication CC-BY-4.0. PMID:36414642

Confocal microscopy showing the spindle structures and distribution of chromosomes in Sept 9 siRNA at 8 h after cold treatment. Spindle and DNA were stained with α‐tubulin‐FITC (green) antibody and DAPI (blue), respectively. ACA stained red. Image from publication CC-BY-4.0. PMID:36354207

Double immunofluorescence of ACA and SYCP3 in 16.5 dpc mouse oocytes. Image from publication CC-BY-4.0. PMID:36429134

Immunolabeling of spreaded WT mouse pachytene spermatocytes positively labeling CREST/Centromere Protein (red, Cat 15-235, 1:20) and SYCP3 (green). Image kindly provided by Fernando Sánchez Sáez and Alberto M. Penás from Universidad de Salamanca, Spain.

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Species Reactivity

Hamster, Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Human

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Data Sheet

SKU: 15-235

Volume: 1 mL

Variant

Price:

$179.00

Quantity:

Product Details

Scientific Background

Purified IgG derived from Human anti-Centromere positive serum (also known as CREST patient serum). Centromere refers to the DNA region where the kinetochore complex assembles, though these terms are often used interchangeably. Anti-Centromere Antibodies (derived from human CREST patient serum) are useful as controls in ANA diagnostics and also reacts with other species including mouse, rat, and hamster.

Format

Adsorptive Chromatography

Concentration

Lot dependent (1-2 mg/mL)

Clonality

Polyclonal

Applications

ICC, IHC

Host Species

Human

Molecular Weight

n/a

Species Reactivity

Hamster, Human, Mouse, Rat

Antibody Registry ID

AB_2939059

Physical State

Liquid

Conjugate

Unconjugated

Quality Control

Serum obtained from an autoimmune patient was purified by protein A chromatography and then tested at a series of dilutions by immunocytochemistry on ethanol-fixed Hep2 cells that were in log-phase growth. The staining pattern obtained was consistent with the pattern expected for anti-centromere staining.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

CREST, Centromere

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Anti-Centromere Protein Antibody

SKU: 15-235

Product Details

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