Polyclonal Antibodies

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa GABAA β3 protein phosphorylated at Ser408/409 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-GABAA Receptor, ß3 (Ser408/409) Antibody
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Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.Mouse auditory neurons expressing a V5-tagged reporter protein were stained with Aves Labs chicken anti-V5 antibody at 1:500. Photo courtesy of Thomas Coate.
Aves Labs Anti-V5 Antibody
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Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diaphragm tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (AP2041; lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide (AX2045).Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 (AP2041) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-Atrogin-1 Antibody
$348
Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (1200 units/100uL lysate overnight).Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Ser1166 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-NMDA NR2B Subunit (Ser1166) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (Cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (Cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41–10 μM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853
PhosphoSolutions Anti-GluR1-Subunit (Ser831) Antibody
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Immunohistochemical staining of Synaptotagmin-1 (green, 1:1000 dilution) and Vimentin (red, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.Immunohistochemical staining of Synaptotagmin (green, 1:1000 dilution) and NFH (red, Rockland, 1:500 dilution) and Vimentin (blue) in a cryostat section through the Cochlear nerve and Organ of Corti in an adult mouse.
Aves Labs Anti-Synaptotagmin-1 Antibody
$481
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.Bar graph showing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.
PhosphoSolutions Anti-Phosphoserine/threonine Antibody
$348
Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. p112-269, red, 1:1000) and total AQP2 (green). Nuclei labeled with DAPI. Magnification 600x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
PhosphoSolutions Anti-Aquaporin 2 (Ser269) Antibody
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Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-AKT (Thr342) Antibody
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Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP (Cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
PhosphoSolutions Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody
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Immunofluoresence of COS-7 cells expressing TPH2-flag using Aves Labs chicken anti-TPH2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-TPH2 signal and anti-flag in transfected cells.Western blotting of TPH2-flag or mock transfected COS-7 cell lysates (10 ug/ml) with Aves Labs chicken anti-TPH2 antibody (5 ug/ml) and detected with anti-mouse HRP.
Aves Labs Anti-TPH2 Antibody
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Immunofluorescence of TREM2-FLAG transfected COS-7 cells using chicken α-TREM2 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.Western blot of rat brain membrane lysate using chicken α-TREM2 showing specific immunolabeling of the endogenous TREM2 at ~35kDa.
Aves Labs Anti-TREM2 Antibody
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Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat. 2027-THSHP, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight. The image was kindly provided by Eugene Dimitrov, Department of Physiology and Biophysics, Rosalind Franklin University.Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
PhosphoSolutions Anti-Tyrosine Hydroxylase Antibody
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Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (Cat. 322-COLT, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (Cat. : 322-COLT, red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
PhosphoSolutions Anti-Collagen 1, alpha 1 telopeptide Antibody
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Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts. Anti-vimentin antibody, 1:1000; HRP-labeled goat anti-chicken IgY, 1:500.Culture of neurosphere cells from an e16 mouse brain immunostained for vimentin immunoreactivity. Vimentin antibody, 1:1000. Secondary, fluorescein-labeled goat anti-chicken antibody (Aves Labs, Cat.No. F-1005), 1:500. Hoda Ilias, Aves Labs.
Aves Labs Anti-Vimentin Protein Antibody
$481
Immunohistochemistry photomicrograph of PLP immunoreactivity (green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. Blue is DAPI nuclear counterstain. Western blot shows a single band in adult mouse brain lysate, 27 ug loaded, 10% gel. Hoda Ilias, Aves Labs.
Aves Labs Anti-Proteolipid Protein (PLP) Antibody
$481
Low-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).Higher-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).
Aves Labs Anti-Integrin alpha-M (CD11b, ΜΑC-1, OX-42 Antigen) Antibody
$481
Immunofluorescence of COS7 cells expressing mKate2 or mRuby using chicken anti-mKate2 antibody (green) and mKate2 autofluorescence (red), showing complete overlap in the signal in transfected cells. The cells were stained using goat anti-chicken IgY FITC secondary antibody (Cat. 80-1000-FITC) and mounted with Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. Western blotting of mock, mKate2, or mRuby transfected COS-7 cell lysates with Aves Labs chicken anti-mKate2 antibody (0.2 ug/ml) and detected with anti-chicken HRP secondary antibody (Cat. 80-1000-HRP). Aves Labs chicken anti-mKate2 recognizes exogenous mKate2 as well as mRuby, another fluorescent protein derived from Entacmaea quadricolor in COS-7 cells.
Aves Labs Anti-mKate2 Antibody
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Immunohistochemical presence of tau in neurofibrillary tangles in cortical neurons of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.Immunohistochemical presence of tau in a neurofibrillary tangle in the remnant of a cortical neuron of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.
Aves Labs Anti-Tau Antibody
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Western blot analysis of human A431 epithelial cells treated with 100 nM calyculin A for 30 min. (lanes 1, 3, 5, & 7) then the blot was treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with polyclonal anti-ERK2 (a.a. 181-195) (lanes 1 & 2), anti-ERK2 (Thr-188) (lanes 3 & 4), anti-ERK1/2 (Thr-202/Tyr-204) (lanes 5 & 6), or monoclonal anti-ERK1 (C-terminal region) (lanes 7 & 8).
PhosphoSolutions Anti-ERK1(T207)/ERK2(T188) [conserved], Phosphospecific Antibody
$348
Western blot of 10 µg of HEK 293 cells showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the C2 splice variant insert (lane 2). 1) HEK cells without NR1 expression; 2) NR1 subunit containing only the C2 Insert; 3) NR1 subunit containing the C1 and C2' insert; 4) NR1 subunit containing the N1 and C2' insert.
PhosphoSolutions Anti-NMDA NR1 Subunit, Splice Variant C2 Antibody
$456
Neurosphere (organoid) cultures of e13 mouse brain were cultured for 2 weeks, and then paraformaldehyde (2%) fixed. After extensive washing, the cultures were incubated with Netrin-1 antibody (Cat. NET; 1:500 dilution), washed, and then treated with fluorescein-labeled goat anti-chicken IgY (Aves Cat. No. F-1005; 1:500 dilution). Hoda Ilias, Aves Labs.
Aves Labs Anti-Netrin-1 Antibody
$481
HeLa cells were subjected to immunofluorescent staining using chicken anti-TOMM20 antibody (visualized in green) and Antibodies Inc mouse anti-Mortalin antibody (75-127) (visualized in red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (AR-6501). The staining revealed a complete overlap between the signal from the chicken anti-TOMM20 antibody and the mouse anti-Mortalin antibody specifically in the cell mitochondria.Western blotting of various cell lysates with chicken anti-TOMM20 antibody (0.2 µg/ml)(1:1000) and detected with anti-chicken HRP. Chicken anti-TOMM20 recognizes endogenous TOMM20 in all the cell lysates at ~16 kDa.
Aves Labs Anti-TOMM20 Antibody
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Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3405).
PhosphoSolutions Anti-MuRF1 (C-terminal region) Antibody
$348

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