Monoclonal Antibodies

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Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with untagged KChIP1, KChIP2, KChIP3, KChIP4 or Kv2.1 plasmid probed with N423/75 (left) or K55/82 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and gene- trap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis).
Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA- tagged Kir6.2 or untagged Kv2.1 plasmid probed with N366/60 (left) or N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or wild-type (WT) or KA2 knockout (KO) mice probed with N279B/27 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Anis Contractor (Northwestern University.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS-1 cells transiently transfected with KCNQ1, KCNQ2, KCNQ3, KCNQ4, KCNQ5 or Kv2.1 plasmids and probed with pure N26A/23.
Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).
Immunofluorescence of COS cells expressing 6xHis/Myc-tagged human VDAC1. Green = N144/14, red = Rabbit anti-Myc, blue = Hoechst nuclear stain.Immunoblot against extracts of COS cells transiently transfected with 6xHis/Myc-tagged Olig2 or untagged Kv2.1 plasmid probed with N144/14 (left), 19E10 (middle) or K89/34 (right) TC supe.
Adult rat brain membrane immunoblot.Lysates of rat cortical neurons treated with the indicated compounds were subjected to ABE to purify palmitoylated proteins. Levels of palmitoyl-AnkG-190 (right blot) and total AnkG (Cat. 75-187) expression in parent lysates (left blot bottom blot) were detected with specific antibodies. Exclusion of NH2OH was used as a control for assay specificity. Image from publication CC-BY-4.0. PMID:36969554
Adult rat cerebellar immunohistochemistry.Representative confocal images of control and α-Syn fibril-treated mouse neurons stained with IP3R1 (Cat. 75-035; magenta) and MAP2 (cyan) with or without treatment of 100 nM UNC-3230. Zoomed-in images show only IP3R1. Image from publication CC-BY-4.0. PMID:37838947
Immunoblot against crude membranes from adult rat brain (RBM), mouse brain (MBM) and wild-type (WT) and ATAT1 knockout (KO) mouse brains probed with N347/42 (left) or N52A/42 (right) TC supe. WT and KO mouse brains courtesy of Andrea Aguilar and Max Nachury (Stanford
NeuroMab Anti-ATAT1 Antibody
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Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/22 (left) or N52A/42 (right) TC supe. Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against isolated cilia and extracts of COS cells transiently transfected with GFP-tagged DNAIC1 or untagged Kv2.1 plasmid probed with UNC 65.56.18.11 (left) or N52A/42 (right) TC supe.Immunolabeling of mouse WT or Kif6 (p.G555fs) mouse ependymal cells at D14 with labeling Dnai1 (Cat. 75-372, green, 1:100, outer dynein arm staining) and Arl13b (magenta, cilia staining). Image from publication, CC-BY-4.0. PMID: 38235522.
Adult mouse brain immunoblot: extracts of brains from wild-type (1), Netrin-G2 knockout (2) and NGL-2 knockout (3) mice were probed with Netrin-G2 antibody (left) or N50/36 (right). Reproduced with permission from 2007 Zhang et al Genes Brain Behav.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2, NGL-3 or untagged Kv2.1 plasmid and probed with N50/36 (left) or K89/41 (right) TC supe.
Anti-Navbeta1 Na+ Channel Antibody
Sliced human breast tumor was stained using Anti-GIT1 antibody (NeuroMab, Cat. No. 75-094, Clone N39/8, 1000x), then followed by anti-Mouse IgG (H+L) secondary antibody, Alexa FluorTM (Invitrogen, A-21425) and DAPI for nuclei. Scale bar 10 μm. Image kindly provided by Songbai Zhang, Karolinska Institutet, Stockholm, Sweden.Adult rat hippocampus immunohistochemistry.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of CV-1 fibroblast cells transfected with VGlut3 (+) or left untransfected (-) and probed with N34/34 TC supe. Image courtesy of Jeff Erickson, Louisiana State University Health Sciences Center.
Adult rat brain immunohistochemistry.Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N25/35 (left) or N52A/42 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).Adult rat hippocampus immunohistochemistry.

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