Monoclonal Antibodies

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Adult rat somatosensory cortex immunohistochemistry.Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with L114/81 (left) or N52A/42 (right) TC supe.
Immunoblot on adult rat brain membrane (RBM) and lysates from Kv1.5-transfected (COS+) and untransfected (COS-) COS cells.Immunofluorescence staining transiently Kv1.5-transfected COS cells.
Adult rat brain membrane immunoblot.Immunoblot on membrane fractions of whole rat (RBM) and mouse (MBM) brain and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Anti-c-FOS AntibodyAnti-c-FOS Antibody
Immunoblot against fractions of adult rat brain membranes (RBM) and extracts of COS or HEK cells transiently or stably expressing different untagged Nav1.X or Kv2.1 plasmid probed with N419/40 (left) or K58/35 (right) TC supe.
Hippocampus Immunohistochemistry from adult rats treated with kainic acid.Immunohistochemistry against cortex.
Immunostaining of spinal cord samples from WT and Gamma-protocadherin KO mice with N159/5 TC supe. Data courtesy of Joshua Weiner, University of Iowa.Adult rat brain membrane immunoblot.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged VAPA, VAPB or untagged Kv2.1 plasmid probed with N479/107 (left) or N86/8 (right) TC supe.Representative images of transfected neurons (white arrow) expressing GCaMP or MitoPAGFP (green) and VAP CRISPR-Cas9 sgRNAs (e) and immunostained for VAP (Cat. 75-496, 1:100; white) show reduced VAP immunofluorescence compared to untransfected, Control neurons (white arrowheads), identified using Map2. Similar results were obtained with VAP shRNA KD. Image from publication CC-BY-4.0. PMID:38177103
Adult mouse brain immunoblot: extracts of cerebella from Ataxin-1 KO and WT mice and probed with N76/3 TC supe. Image courtesy of Drs Paymaan Jafad-Nejad and Huda Zoghbi, Baylor College of Medicine.Adult rat hippocampus immunohistochemistry.
Immunoblot against membrane fractions from adult rat brain (RBM) and heart (RHM) and Kcnv2-S2 mouse wild-type (+/+) and higher-expressing transgenic (Tg/+) brains probed with N448/88 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Jennifer Kearney (Northwestern).
Adult rat whole brain immunohistochemistry.Adult rat brain membrane immunoblot.
Immunostaining of mouse brain cortex identifying the calcium channels along axons stained with Anti-Cav1.3 Ca2+ Channel Antibody (Cat. 75-080, 1:500, green) and nuclei are labeled with DAPI (blue). Image kindly provided by Kaveh Moradi, Virginia Commonwealth University.Adult rat hippocampus immunohistochemistry.
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N29/29 (left) or N52A/42 (right) TC supe.Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N29/29 (left) and N52A/42 (right) TC supe.
NeuroMab Anti-VGlut2 Antibody
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Adult rat brain membrane immunoblot.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against brain membranes from adult rat (RBM) or from Slo2.1 knockout (MBM-Slo2.1 KO) or wild-type (MBM-WT) mice probed with N11/33 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Chris Lingle (Washington University).Adult rat hippocampal immunohistochemistry.
Surface immunofluorescence (no detergent permeabilization) of cultured rat hippocampal neuron transfected with HA-tagged Kv2.1 plasmid. Red = K39/25. Scale bar = 20 m. Reproduced from 2000 Lim et al Neuron (PMID 10719893).Adult rat brain membrane immunoblot
Immunoprecipitation analysis of TRPC5 protein in brain microsomes extracted from wild-type (WT) and TRPC5 knockout (KO) littermates. Courtesy of Dr. David Clapham (HHMI/Harvard Medical School).Adult rat hippocampal immunohistochemistry.
Adult rat brain membrane immunoblot.Adult rat hippocampus immunohistochemistry.
Cell-surface-expressed ADAM22 (F, magenta) and cell-surface-bound LGI1 (G, magenta) were live-labelled. After fixation and permeabilization, ADAM22 (total) was stained (Cat. 75-083; green), with nuclear DNA (blue, F and upper panel in G). ADAM22 S905F is on the cell surface binds to LGI1 as the wild-type. When ADAM22 C401Y is expressed, no cell-surface-bound LGI1 is detected. White squares are total expression of ADAM22 (F) and LGI1–FLAG (lower panel in G, blue). CC-BY-4.0. PMID:37953841Immunoblot versus membranes from postnatal day 8 wild-type (wt) and ADAM22-knockout (ko) mice and probed with N46/30 (left) and N57/2 (right). Data courtesy of Dies Meijer, Erasmus University Rotterdam.

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