Anti-LRRK2/Dardarin, N3 (Non-Mouse-Reactive) Antibody (N231B/34)

Name: Anti-LRRK2/Dardarin, N3 (Non-Mouse-Reactive) Antibody
Brand: NeuroMab
SKU: 75-266
Price: 329.0 USD
Availability: InStock

5 Citations

Our Anti-Dardarin/LRRK2, N3 (non-mouse-reactive) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N231B/34. It is KO validated, detects human, mouse, and rat Dardarin/LRRK2, N3 (non-mouse-reactive), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against lysates of wildtype (WT) and LRRK2 knockout (KO) rat brains probed with N231B/34 (top) and anti- actin antibody (bottom). Reproduced with permission from the Proceedings of the National Academy of Sciences of the United States of America (2014 Daher et al, PMID 24927544).

Adult rat brain membrane immunoblot.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-266

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Dardarin/LRRK2, N3 (non-mouse-reactive)

Scientific Background

LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N231B/34

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

LRRK2 PARK8

Molecular Weight

>200 kDa

Immunogen

Fusion protein amino acids 841-960 of human LRRK2 (accession number Q5S007) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_11000724

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

UniProt ID

Q5S007

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
26758690	1:200	Belluzzi, E., et al. 2016. LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate.. Molecular Degeneration, 1.
26365310	1:50	Choi, I., et al. 2015. LRRK2 G2019S mutation attenuates microglial motility by inhibiting focal adhesion kinase.. Nature Communications, 8255.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
23560750	1:1000	Davies, P., et al. 2013. Comprehensive characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies.. Biochemical Journal, 101-13.

Immunohistochemistry: Rat
PMID	Dilution	Publication
24927544	not listed	Daher, J.P., et al. 2014. Abrogation of α-synuclein-mediated dopaminergic neurodegeneration in LRRK2-deficient rats.. PNAS USA, 9289-9294.

Western Blot: Mouse
PMID	Dilution	Publication
23560750	1:1000	Davies, P., et al. 2013. Comprehensive characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies.. Biochemical Journal, 101-13.
24427314	not listed	Dorval, V., et al. 2014. Gene and MicroRNA transcriptome analysis of Parkinson''s related LRRK2 mouse models.. PLoS One, e85510.

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Anti-LRRK2/Dardarin, N3 (Non-Mouse-Reactive) Antibody (N231B/34)

SKU: 75-266

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-LRRK2/Dardarin, N3 (Non-Mouse-Reactive) Antibody

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