Monoclonal Antibodies

3762 products

Monoclonal antibodies are generated from hybridoma clones and are powerful research tools for analyzing proteins to understand biological and cellular functions.

We have a broad portfolio of monoclonal antibodies, including the NeuroMab line, which was developed in collaboration with the UC Davis – NIH facility. These highly-cited, highly-validated antibodies have been trusted by the neuroscience research community for years. Knockout validation guarantees target specificity and production from hybridoma clones provides batch-to-batch consistency and long-term supply.

Analysis of multiple proteins within the same tissue sample, using immunofluorescence is key to advancing research. To support the multiplexing field we provide fluorescent conjugates which include 490, 550, 594 and 650. Our mouse monoclonal conjugates continue to grow in popularity.

Showing 97 - 120 of 3762 products

Immunofluorescence staining of unpermerabilized COS cells transiently transfected with GFP- tagged GluN3A/NR3a and GluN1/NR1 plasmids with N416/40 (red). Images courtesy of Sonia Marco Martinez and Isabel Perez- Otano (University of Navarra).

Immunoblot against crude membranes from neonatal or adult rat brain (RBM) and extracts of COS cells transiently transfected with untagged GluN3A/NR3A or Kv2.1 plasmid probed with N416/40 (left) or K35/40 (right) TC supe.

NeuroMab Anti-GluN3A/NR3A Antibody

$337

Hippocampus Immunohistochemistry from adult rats treated with kainic acid.

NeuroMab Anti-Npas4 Antibody

$337

Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right).

NeuroMab Anti-Synaptotagmin-7 Antibody

$337

Immunoblot versus lysates of total brain from HCN3 knockout (KO) and wild-type (WT) mice probed with N141/28. Data courtesy of Martin Biel (Ludwig-Maximilians Universität München) and reproduced from the Journal of Biological Chemistry (2013 Cao-Ehlker et al, PMID 23382386).

Adult rat hippocampus immunohistochemistry.

NeuroMab Anti-HCN3 Antibody

$337

Immunoblot versus rat brain low-speed pellet (LSP), mouse brain membranes (MBM) and wild-type (WT) and LRRK2 KO mouse samples probed with N138/6 (left) and K89/41 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson (Johns Hopkins University).

NeuroMab Anti-LRRK2/Dardarin, N-Terminus Antibody

$337

Array tomography immunofluorescence volume reconstruction of 9 serial LRWhite-embedded 70 nm sections from adult mouse cortex layer 6 with L121/25 (green), rabbit Synapsin (Cell Signaling 5297, red). Image courtesy of Kristina Micheva (Stanford).

NeuroMab Anti-nNOS/NOS1 Antibody

$337

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/83 (green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford).

Adult rat cerebellum immunohistochemistry.

NeuroMab Anti-Gephyrin Antibody

$337

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with GAD65/67 recombinant chicken mAb (green) (Cat. 78-508) at 1:100 dilution. The stained sections were mounted with FluoroshieldTM mounting medium (Cat. AR-6505). DAPI (blue) shows cell nuclei. Anti-GAD65/67 stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 100X.

Western blotting of rat brain homogenate with Antibodies Incorporated anti-GAD65/67 mouse mAb (Cat. 75-508) and Aves Labs anti-GAD65/67 recombinant chicken mAb (Cat. 78-508) antibodies. The mouse mAb and chicken mAb were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. Aves Labs recombinant chicken anti-GAD65/67 mAb recognizes endogenous GAD65/67 with similar sensitivity as the anti-GAD65/67 NeuroMab in the rat brain homogenate at ~65-67 kDa.

Aves Labs Anti-GAD65/67 Recombinant Chicken Chimeric mAb

From $144

Representative image of 4% paraformaldehyde fixed free-floating rat brain sections stained with anti-c-FOS recombinant chicken monoclonal antibody (red) at 1:1000 dilution and co-stained with anti-GFAP rabbit polyclonal antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-c-FOS specifically stains only the nuclei of the spontaneously active neurons. Image courtesy of EnCor Biotechnology.

Western blotting of serum starved (24 h) and serum stimulated (2 h) HeLa cells using Aves Labs anti-c-Fos recombinant chicken monoclonal antibody (Cat. 78-506). The chicken mAb was detected with anti-chicken-HRP (Cat. 80-1000-HRP). Aves Labs Recombinant Chicken anti-c-Fos mAb recognizes endogenous c-Fos in the serum stimulated HeLa cells at ~65 kDa.

Aves Labs Anti-c-FOS Recombinant Chicken Chimeric mAb

From $144

Stable cell immunoblot: extracts of HEK cells stably-expressing Flag-tagged Cav3.2, Cav3.1 or untagged Kv2.1 plasmid and probed with N55/10 TC supe (left) or Rabbit anti-Flag (right).

Immunohistochemistry of coronal brain sections from WT and Cav3.2 KO mice. Reproduced with permission from Mala Shah (University College London, England, UK) and Nature Neuroscience (2011, Huang et al., PMID 21358644).

NeuroMab Anti-Cav3.2 Ca2+ Channel Antibody

$337

$Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).$

Immunolabelling showing α1-/α5-containing GABAA receptors demonstrated synaptic localizations in a primary hippocampal neuronal culture. Distributions of α1 and α5 (Cat. 75-401, 1:1000) subunits (A,B) were co-stained with the inhibitory presynaptic marker protein vGat (C). (D) The α1 subunit was mostly synaptically localized, opposing vGat, whereas α5 was distributed at both the soma and synapse and often showed co-localization with α1-vGat puncta at the synapse (E,F). CC-BY-4.0. PMID:38201218

NeuroMab Anti-GABA-A-R-Alpha5 Antibody

$337

Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.

NeuroMab Anti-Beta4-Spectrin Antibody

$394

Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/1 (red) and Hoechst nuclear stain (blue).

Adult rat whole brain immunohistochemistry.

NeuroMab Anti-Kv2.2 Potassium Channel Antibody

$337

Immunoblot against crude brain membrane preparations from adult rat (RBM) or extracts of COS cells transiently transfected with Myc- tagged LRRTM1, LRRTM2 or untagged Kv2.1 plasmid probed with N209C/35 (left), K89/34 (middle) or N52A/42 (right) TC supe.

NeuroMab Anti-LRRTM2 Antibody

$337

Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.

Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.

NeuroMab Anti-Olig1 Antibody

From $142

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Kir2.1 or untagged Kv2.1 plasmids and probed with N112B/14 (left) and K89/41 (right) TC supe.

NeuroMab Anti-Kir2.1 K+ Channel Antibody

$337

Immunoblot against crude brain membranes from adult rat (RBM) and wild-type (WT) and Kv4.2 knockout (KO) mice probed with L28/4 (left) or N52A/42 (right) TC supe.

NeuroMab Anti-Kv4.2 Potassium Channel Antibody

$337

array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/4 (green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford).

NeuroMab Anti-Gephyrin Antibody

$337

Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and Caspr2 knockout mice. Caspr KO sample courtesy of Drs. Ido Horresh and Ori Peles, The Weizmann Institute of Science.

NeuroMab Anti-CASPR2 Antibody

From $142

Immunofluorescence staining of adult rat optic nerve with K73/20 (red) and βIV-spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).

NeuroMab Anti-Contactin/F3 Antibody

$337

NeuroMab Anti-SAP97 Antibody

$337

Representative image of 4% paraformaldehyde fixed free-floating rat hippocampus sections stained with Aves Labs anti-Parvalbumin recombinant chicken monoclonal antibody (red, Cat No. 78-479) at 1:1000 dilution and co-stained with anti-Iba1 rabbit polyclonal antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-Parvalbumin specifically stains the GABAnergic interneurons in the cerebellum. Image courtesy of EnCor Biotechnology.

Representative image of 4% paraformaldehyde fixed free-floating rat cerebellum sections stained with Aves Labs anti-Parvalbumin recombinant chicken monoclonal antibody (Cat No. 78-479) at 1:1000 dilution. Anti-Parvalbumin specifically stains the Purkinje cells and other neurons in the molecular layer of the cerebellum. Magnification 400X. Image courtesy of EnCor Biotechnology.

Aves Labs Anti-Parvalbumin Recombinant Chicken Chimeric mAb

From $144

Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.

Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3 (Cat. 583-FOX3, green, 1:1000) and Anti-MAP2 (Cat. 1100-MAP2, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.

PhosphoSolutions Anti-FOX3 (NeuN) Antibody

From $164

Transfected cell immunoblot: COS cells transiently transfected with Ataxin-1 and Kv2.1 plasmids and probed with N76/8 TC supe.

Adult rat brain immunoblot: extracts of cerebella from Ataxin-1 KO and WT mice and probed with N76/8 (left) or K89/41 (right) TC supe.

NeuroMab Anti-Ataxin-1, 11NQ Antibody

$337

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