Monoclonal Antibodies

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Adult rat brain membrane immunoblotRepresentative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat. 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma- protocadherin-C3 and –A3 and untagged Kv2.1 plasmids and probed with N174B/27 (left) and N159/5 (right) TC supe.
Adult rat brain membrane immunoblot.
Crude lysate of 7-day old zebrafish larvae immunoblot.Isolated goldfish cone immunohistochemistry. Data courtesy of Diane Henry-Vanisko and Gary Matthews.
Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cortex layer 2/3 with L115/13 (green), GABA (red) and DAPI (blue). Volume reconstruction of 35 serial 70 nm sections show two NPY-positive neurons with varying levels of GABA reactivity, as well as some NPY-reactive axonal varicosities. Images courtesy of Kristina Micheva (Stanford).Adult rat cortex immunohistochemistry.
Cerebellum immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot on adult rat brain membrane (RBM) and lysates from Kvβ1.1 -transfected (COS+) and untransfected (COS-) COS cells.
Adult rat basal forebrain immunofluorescence with antigen retrieval via sodium citrate pretreatment with K37/89 (red) and Kv2.1 rabbit (green). Image courtesy of Kaori Misonou and Hiroaki Misonou (University of Maryland, now at Doshisha University, Japan).Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with Myc-tagged Kv2.2short, GFP-tagged Kv2.2long or untagged Kv2.1 plasmid probed with K37/89 (left), K89/34 (middle) or N52A/42 (right) TC supe.
Anti-Mortalin/GRP75 Antibody FL650 Conjugate
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/12 (left) or N52A/42 (right) TC supe. Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Immunofluorescence labeling of adult rat hippocampus with N450/53 TC supe (red) and Hoechst nuclear stain (blue).Anti-BDNF Antibody
Adult rat pontine nucleus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Immunoblot against crude C. elegans worm extracts and brain membranes from adult rat (RBM) probed with N72/16 TC supe.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2, Shank3 or Kv2.1 plasmids and probed with N69/46 TC supe (left panel) or a monoclonal lane loading control (right panel).
NeuroMab Anti-Shank3 Antibody
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Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/21 (left) or N52A/42 (right) TC supe.II Confocal images of Dr-Vsp immunostaining in a 7-dpf wild-type zebrafish larva (sagittal section). Dr-Vsp is highly expressed in enterocytes at the posterior part of the mid-intestine, which are defined as lysosomerich enterocytes (LREs). Image from publication, CC-BY-4.0, PMID: 36088390
Whole brain immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).
NeuroMab Anti-BAF53b Antibody
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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N278/19 (left) and N86/8 (right).Adult rat brain membrane immunoblot.

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