Ships: 1-2 business days
- Prepare samples and controls
- Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.
- Reconstitute FLICA with 50 µL DMSO.
- Dilute FLICA 1:5 by adding 200 µL PBS.
- Add diluted FLICA to each sample at 1:30 (e.g., add 10 µL to 290 µL of cultured cells).
- Incubate approximately 1 hour.
- Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells.
- If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody.
- If desired, fix cells.
- Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm.
If working with adherent cells, please see the manual for additional protocols.
Kit 93: 25 Tests
FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 1 vial, #653
10X Apoptosis Wash Buffer, 15 mL, #635
Fixative, 6 mL, #636
Propidium Iodide, 1 mL, #638
Hoechst 33342, 1 mL, #639
Kit Manual
Kit 94: 100 Tests
FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 4 vials, #653
10X Apoptosis Wash Buffer, 60 mL, #634
Fixative, 6 mL, #636
Propidium Iodide, 1 mL, #638
Hoechst 33342, 1 mL, #639
Kit Manual
Product Specific References
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39335166 | Chia, W.T., et al. 2024. Okanin Inhibits Cell Growth and Induces Apoptosis and Pyroptosis in Oral Cancer. Cancers, . |
39199694 | Finiuk, N., et al. 2024. The Proapoptotic Action of Pyrrolidinedione-Thiazolidinone Hybrids towards Human Breast Carcinoma Cells Does Not Depend on Their Genotype. Cancers, . |
38972660 | Li, J, et al. 2024. Mode of cell death in the penile cavernous tissue of type 1 diabetes mellitus rats. The journal of sexual medicine, 652-662. |
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