Anti-Pan-GRK (N145/20)

Name: Anti-Pan-GRK
Brand: NeuroMab
SKU: 75-179
Price: 337.0 USD
Availability: InStock

Our mouse Pan-GRK monoclonal antibody from NeuroMab is produced in-house from clone N145/20. It detects human, mouse, and rat Pan-GRK and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Immunoblot against adult rat brain membrane (RBM) and extracts of COS cells transiently transfected with untagged GRK5, GRK6 or Kv2.1 plasmid and probed with N145/20 TC supe.

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N145/20 (left) or N52A/42 (right) TC supe.$

Adult rat brain membrane immunoblot.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-179

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Pan-GRK

Scientific Background

G protein-coupled receptor (GPCR) kinases (GRKs), are important regulators of GPCR function and mediate receptor desensitization, internalization, and signaling. Several non-GPCR proteins also serve as GRK substrates, and GRKs have been shown to have kinase-independent functions through regulation of receptors and effectors. These proteins are members of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. GRKs phosphorylate activated forms of G protein-coupled receptors to regulate their signaling.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N145/20

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

Grk5 Gprk5 Grk6 Gprk6

Molecular Weight

70 kDa

Immunogen

Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5 (accession number Q8VEB1) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2232788

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.107

Conjugate

Unconjugated

Specificity

Cross-reacts with GRK6

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Gprk5; G protein-coupled receptor kinase 5; g protein-coupled receptor kinase GRK5; EC 2.7.11.16; G protein-coupled receptor kinase GRK5 G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6

UniProt ID

Q8VEB1
UniProt Summary: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).

NCBI Gene ID

14773

Shipping

Shipped on ice packs

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Anti-Pan-GRK (N145/20)

SKU: 75-179

Product Details

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