Anti-Doc2B Antibody (N150/21)

Name: Anti-Doc2B Antibody
Brand: NeuroMab
SKU: 75-214
Price: 337.0 USD
Availability: InStock

Our mouse Doc2b monoclonal antibody from NeuroMab is produced in-house from clone N150/21. It detects mouse and rat Doc2b and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat hippocampus immunohistochemistry.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with GFP-tagged Doc2b or untagged Kv2.1 plasmids and probed with N150/21 (left), N86/8 (middle) and K89/41 (right).

Adult rat hippocampus immunohistochemistry.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-214

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Doc2b

Scientific Background

Double C2-like domain-containing protein beta or Doc2b is encoded by the gene DOC2b. Doc2b functions as a calcium sensor which positively regulates soluble NSF attachment receptor (SNARE) dependent fusion of vesicles with membranes. Doc2b binds phospholipids and may act at the priming stage of membrane fusion by modifying membrane curvature to stimulate fusion. Doc2b is expressed in pancreatic cells, endocrine cells and brain, where it is highly expressed in neurons. (Friedrich et al.Neurosci., July 2, 2008 • 28(27):6794 – 6806)

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N150/21

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Doc2b

Molecular Weight

45 kDa

Immunogen

Fusion protein amino acids 1-216 (N-terminus) of rat Doc2b (accession number P70610) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315830

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Doc2a

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

double C2-like domain-containing protein beta; double C2; Doc2-beta; beta; double C2-like domains

UniProt ID

P70610
UniProt Summary: Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes.

NCBI Gene ID

81820

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Anti-Doc2B Antibody (N150/21)

SKU: 75-214

Product Details

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