Anti-Mitofusin-2 Antibody (N153/5)

Name: Anti-Mitofusin-2 Antibody
Brand: NeuroMab
SKU: 75-173
Price: 337.0 USD
Availability: InStock

Our mouse Mitofusin-2 monoclonal antibody from NeuroMab is produced in-house from clone N153/5. It detects mouse and rat Mitofusin-2 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat hippocampus immunohistochemistry.

Immunoblot versus crude cardiac mitochondrial preps from WT, Mfn1 KO or Mfn2 KO mice and probed with N153/5 TC supe (top) and Porin antibody (bottom). Data courtesy of Dr. Suzanne Hoppins, Dr. Laura Lackner and Dr. Jodi Nunnari, UCDavis.

Adult rat hippocampus immunohistochemistry.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-173

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Mitofusin-2

Scientific Background

Mitofusin 2, or transmembrane GTPase Mfn2 (MFN2) is a mitochondrial membrane protein with GTPase activity that functions to aid mitochondrial clustering and fusion. It may also play a role in mitochondrial metabolism. It is expressed in the outer membrane of mitochondria. MFN2 is expressed in many tissues including the heart, skeletal muscle, tongue, and brain. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI,

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N153/5

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Mfn2 Kiaa0214 Marf

Molecular Weight

90 kDa

Immunogen

Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2 (accession number Q80U63) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2297598

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.101

Conjugate

Unconjugated

Specificity

No cross-reactivity against Mitofusin-1

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

D630023P19Rik; Mitofusin-2; EC 3.6.5.-; HSG protein; Hypertension-related protein 1; hypertension related protein 1; Mitochondrial assembly regulatory factor; transmembrane GTPase MFN2

UniProt ID

Q80U63
UniProt Summary: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains. Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.

NCBI Gene ID

170731

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Mitofusin-2 Antibody (N153/5)

SKU: 75-173

Product Details

Bulk Order Anti-Mitofusin-2 Antibody

Related Products

Recently Viewed