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Immunocytochemical labeling of L1CAM in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-L1CAM (LM0231). The antibody was detected using goat anti-mouse Ig DyLight® 594.Representative Standard Curve using mouse monoclonal antiL1CAM (LM0231) for ELISA capture of human recombinant L1CAM protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Immunoblot against crude membrane fractions from whole rat (RBM) or mouse (MBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N84/37 (left) or N52A/42 (right) TC supe.
Native western blot image of human laminin isoforms: laminin 521 (α5β2γ1), laminin 121 (α1β2γ1), laminin 221 (α2β2γ1), laminin 332 (α3β3γ2), laminin 511 (α5β1γ1), laminin 411 (α4β1γ1), as well as human A431, A549, and NCI-H2052 cells. The blot was probed with mouse monoclonal anti-Laminin β2/γ1 subunit (LM0461) at 1:1000.Immunocytochemical labeling of laminin β2/γ1 subunits in aldehyde fixed and NP-40 permeabilized human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Laminin β2/γ1 subunits (LM0461). The antibody was detected using goat anti-mouse DyLight® 594.
Immunohistochemistry with antigen retrieval via citrate pretreatment.Adult rat hippocampus immunohistochemistry under standard protocol.

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