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Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-β-Dystroglycan (lanes 1 & 2) and anti-β-Dystroglycan (Tyr-892) (lanes 3 & 4).

PhosphoSolutions Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody

$340

Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).

Immunocytochemical labeling of β-tubulin in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-β-Tubulin (TP1781). The antibody was detected using goat anti-rabbit DyLight® 594.

PhosphoSolutions Anti-β-Tubulin (central region) Antibody

$285

Immunocytochemical labeling in C2C12 cells using anti-β-Tubulin (TM1541) monoclonal antibody and anti-β-Tubulin (Ser-172) polyclonal antibody. The specificity of the binding for the latter antibody was demonstrated by using the antibody in the presence of phospho-β-Tubulin (Ser-172) peptide (TX1725).

PhosphoSolutions Anti-β-Tubulin (Ser-172), Phosphospecific Antibody

$340

PhosphoSolutions Anti-β-Tubulin Antibody

$285

Western blot analysis of mouse brain. The blot was probed with anti-unphosphorylated βIII-Tubulin (Ser-444) (lanes 1-3) and anti-βIII-Tubulin (C-terminus) (lanes 4-6) polyclonal antibodies. Both antibodies were used in the presence of unphosphorylated βIII-Tublin (Ser-444) peptide (lanes 2 & 5; TX1815) and phospho-βIII-Tublin (Ser-444) peptide (lanes 3 & 6; TX1695).

Immunocytochemical labeling in chick dorsal root ganglion neurons using anti-Cofilin (N-terminus; CP1131), anti-Cofilin (Ser-3; CP1151), anti-βIII-Tubulin (C-terminus; TP1691) and anti-β-Tubulin (TM1541) antibodies. (Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky).

PhosphoSolutions Anti-βIII-Tubulin (C-terminus) Antibody

$285

PhosphoSolutions Anti-βIII-Tubulin Antibody

$340

Western blot analysis of anti-γ-Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with γ-Catenin (a.a. 545-555), γ-Catenin (Tyr-550) or γ-Catenin (C-terminal) antibodies. The anti-γ-Catenin (Tyr-550) was used in the presence of γ-Catenin (Tyr-550) (lane 5) or γ-Catenin (Tyr-644) (lane 6) peptides.

PhosphoSolutions Anti-γ-Catenin (C-terminal region) Antibody

$285

Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal γ-Catenin (CM1111) or rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.

PhosphoSolutions Anti-γ-Catenin (Tyr-550), Phosphospecific Antibody

$340

Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3) then the blot was treated with lambda phosphatase (lanes 2 & 4). The blots were probed with either mouse monoclonal anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2) or rabbit polyclonal anti-δ1-Catenin (Thr-916) (lanes 3 & 4).

Immunocytochemical labeling of δ1-Catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), or δ1-Catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.

PhosphoSolutions Anti-δ1-Catenin (central region) Antibody

$370

Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-δ1-Catenin (Tyr-96) (lanes 3 & 4), anti-δ1-Catenin (Tyr-228) (lanes 5 & 6), anti-δ1-Catenin (Tyr-280) (lanes 7 & 8) or anti-δ1-Catenin (Tyr-302) (lanes 9 & 10).

PhosphoSolutions Anti-δ1-Catenin (Tyr-228), Phosphospecific Antibody

$370

ImmunoChemistry Technologies Antibody Coating Buffer, 5X

From $55.90

ImmunoChemistry Technologies Antibody Dilution Buffer with BSA (Immunoglobulin Free)

From $60

ImmunoChemistry Technologies Antibody-Sandwich ELISA Development Kit

$669

Figure 1. Optimization of double-antigen sandwich ELISA. (a) Capture (0.5 to 5.0 µg/mL) and detection antigen (0.2 (green), 0.4 (light blue), 0.8 (yellow), and 1.6 (purple)) were used to optimize capture and detection antigen (N protein). Capture antigen was diluted with Antigen Coating Buffer. From Figure 1a of Cordero-Ortiz, M, et al. (2023) Animals (Basel) 13, 3487 doi: 10.3390/ani13223487. Used under CC License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.

ImmunoChemistry Technologies Antigen Coating Buffer, 5X

From $55.90

Figure 1. Equalize the matrix with an assay diluent

ImmunoChemistry Technologies Antigen-Down Assay Diluent

From $59

Figure 1. In Antigen-Down-ELISAs, a Target Antigen coated onto the ELISA plate surface is used to quantify specific antibody concentrations present within samples. When the sample is added, the antigen on the plate is bound by antibodies from the sample, which are then retained in the well. A species-specific antibody labeled with HRP is added, followed by substrate, a stop solution is added, and then the absorbance is read. The higher the signal, the more antibodies there are in the sample.

Figure 2. Initial Assay Feasibility Plate Map. Wells are coated with 100 µL per well of either 1 µg/mL (light gray) or 4 µg/mL (dark gray) antigen coating solution, or Antigen Coating Buffer blank well controls (white). A known Positive Control is diluted out via a series of 1:2 dilutions and added to six sections of the plate. After the antibody capture step, plates are incubated with three different dilutions of the HRP-IgG conjugate, washed, and developed with TMB substrate. See protocol.

ImmunoChemistry Technologies Antigen-Down ELISA Development Kit

$590

ImmunoChemistry Technologies Antigen-Down HRP Conjugate Stabilizer, 5X

From $70.30

Figure 1. U937 cells were stimulated with staurosporine for 5 hours, and then assayed for caspase 3/7 activity following the kit protocol.

ImmunoChemistry Technologies Apo 3/7 HTS Assay Kit

From $320

Figure 1. Jurkat Cells were stimulated with staurosporine (B) or DMSO (A) for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with anti-active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti-Rabbit antibody.

ImmunoChemistry Technologies Apo Active FITC - Caspase 3 Detection Kit

$522

Figure 1. Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti-active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti-Rabbit antibody.

Figure 2. Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti-active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti-Rabbit antibody.

ImmunoChemistry Technologies Apo Active PE - Caspase 3 Detection Kit

$522

PhosphoSolutions Argonaute 2 Phospho-Regulation Antibody Kit

$459

PhosphoSolutions Arp2/3 Complex Antibody Kit

$459

PhosphoSolutions Arp2/3 Complex Regulation Antibody Kit

$459

ImmunoChemistry Technologies Assay Diluent Optimization Pack

$200.30

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