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Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.

Bar graph showing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.

PhosphoSolutions Anti-Phosphoserine/threonine Antibody

$348

Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. The blot was untreated (lane 1) or treated with lambda phosphatase (lanes 2), then probed with anti-Phosphothreonine (PP4641) at 1:1000.

Immunocytochemical labeling of phosphothreonine upregulation in control (left) or calyculin A-treated HeLa cells (right). The cells were labeled with rabbit polyclonal anti-Phosphothreonine (PP4641). The antibody was detected using goat anti-rabbit DyLight® 594.

PhosphoSolutions Anti-Phosphothreonine Antibody

$292

Western blot of HeLa cells treated with pervanadate (1 mM) for 30 min. Phosphotyrosine containing proteins were immunoprecipitated with rabbit polyclonal anti-Phosphotyrosine:Agarose (Lane 1) or with no antibody agarose beads (Lane 2), and blots were made that included the whole lysate (Lane 3). The blots were probed with mouse monoclonal anti-Phosphotyrosine (PM3751) to detect phosphotyrosine containing proteins.

Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

PhosphoSolutions Anti-Phosphotyrosine Antibody

$292

Bar graph showing rabbit polyclonal anti-Phosphotyrosine (PP2221) binding to a variety of phosphotyrosine containing peptides, but no binding to unphosphorylated peptide (beta-Catenin (a.a. 29-45).

PhosphoSolutions Anti-Phosphotyrosine Antibody

$292

NeuroMab Anti-PhyH/PAHX Antibody

$337

NeuroMab Anti-PhyH/PAHX Antibody (BSA and Azide free)

$486

NeuroMab Anti-PhyH/PAHX Antibody Biotin Conjugate

$543

NeuroMab Anti-PhyH/PAHX Antibody FL490 Conjugate

$486

NeuroMab Anti-PhyH/PAHX Antibody FL550 Conjugate

$486

NeuroMab Anti-PhyH/PAHX Antibody FL594 Conjugate

$486

NeuroMab Anti-PhyH/PAHX Antibody FL650 Conjugate

$486

Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI3 Kinase, p85 (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).

Immunocytochemical labeling of PI3 Kinase p85 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI3 Kinase p85 (C-terminal region) antibody (PM2531), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-PI3 Kinase, p85 (C-terminal region) Antibody

$379

$Immunoblot against membrane fractions from adult rat brain (RBM) and hippocampi (MHM) from wild-type (WT) and PICK1 knockout (KO) mice probed with L20/8 TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).$

Adult rat hippocampus immunohistochemistry.

NeuroMab Anti-PICK1 Antibody

$337

NeuroMab Anti-PICK1 Antibody (BSA and Azide free)

$486

NeuroMab Anti-PICK1 Antibody Biotin Conjugate

$543

NeuroMab Anti-PICK1 Antibody FL490 Conjugate

$486

NeuroMab Anti-PICK1 Antibody FL550 Conjugate

$486

NeuroMab Anti-PICK1 Antibody FL594 Conjugate

$486

NeuroMab Anti-PICK1 Antibody FL650 Conjugate

$486

Simple Western of HeLa lysate showing specific immunolabeling of the ~ 56 kDa PINK1 protein.

Western Blot of C-terminally V5His-tagged human PINK1 or N-terminally myc-tagged human PINK1 expressed in HEK293T cells showing specific immunolabeling of PINK1.

PhosphoSolutions Anti-Pink1 Antibody

$456

Adult rat brain low-speed pellet immunoblot.

Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

NeuroMab Anti-Pink1 PTEN Induced Putative Kinase 1 Antibody

$337

NeuroMab Anti-Pink1 PTEN Induced Putative Kinase 1 Antibody

$337

NeuroMab Anti-Pink1 PTEN Induced Putative Kinase 1 Antibody (BSA and Azide free)

$486

NeuroMab Anti-Pink1 PTEN Induced Putative Kinase 1 Antibody (BSA and Azide free)

$486

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