ICC IHC WB
Potassium two pore domain channel subfamily K member 3 is encoded by the gene KCNK3. KCNK3 is a member of the two pore domain potassium channel (TC 1.A.1.8) family. KCNK3 is a pH dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from the potassium ion concentration on either side of the membrane, with it functioning as an outward rectifier when the external potassium concentration is low, and it functioning as an internal rectifier when the external potassium concentration is high. KCNK3 is expressed in the heart, lung, brain, liver, kidney, and skeletal muscle. Diseases associated with KCNK3 include Pulmonary Hypertension Primary 4 and Heritable Pulmonary Arterial Hypertension.
Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1 (also known as Potassium channel subfamily K member 3, TWIK-related acid-sensitive K(+) channel 1, Two pore potassium channel KT3.1, Cardiac two pore background K(+) channel, KCNK3 and cTBAK-1, accession number O54192)
Mouse: 96% identity (156/161 amino acids identical)
Human: 76% identity (163/161 amino acids identical)
<30% identity with TASK3
Does not cross-react with TASK3
Potassium channel subfamily K member 3 (Acid-sensitive potassium channel protein TASK-1) (TWIK-related acid-sensitive K(+) channel 1) (Two pore potassium channel KT3.1) (Two pore K(+) channel KT3.1)
Kcnk3 Task Task1
TC Supernatant: AB_2315941
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.
Citations and References
- Dadi PK1, Luo B, Vierra NC, Jacobson DA.. (2015), 'TASK-1 Potassium Channels Limit Pancreatic α-Cell Calcium Influx and Glucagon Secretion..' Mol Endocrinol.. 10.1210/me.2014-1321.
- Dadi PK1, Vierra NC, Jacobson DA.. (2014), 'Pancreatic β-cell-specific ablation of TASK-1 channels augments glucose-stimulated calcium entry and insulin secretion, improving glucose tolerance..' Endocrinology. 10.1210/en.2013-2051.