ICC IHC WB
Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 12 differs from classical synaptotagmins in that it does not bind Ca(2+). In synaptic vesicles, synaptotagmin-12 forms a complex with synaptotagmin-1 that prevents synaptotagmin-1 from interacting with SNARE complexes (Maximov A. et al., 2007).
Fusion protein amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12 (also known as Syt12, Synaptotagmin XII, SytXII, Synaptotagmin-related gene 1 protein, Srg1 and Sytr1, accession number Q920N7)
Rat: 100% identity (88/88 amino acids identical)
Human: 95% identity (84/88 amino acids identical)
>30% identity with Synaptotagmin-6 (and other Synaptotagmins)
Does not cross-react with Synaptotagmin-6 (or other Synaptotagmins)
Synaptotagmin-12 (Synaptotagmin XII) (SytXII)
TC Supernatant: AB_2315939
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
45 kDa (plus others)
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.
Citations and References
- Ramachandran B1, Ahmed S, Zafar N, Dean C.. (), 'Ethanol inhibits long-term potentiation in hippocampal CA1 neurons, irrespective of lamina and stimulus strength, through neurosteroidogenesis..' Hippocampus.. 10.1002/hipo.22356.