Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N277/7 (left) and N86/8 (right).
Synaptotagmin-12 (Synaptotagmin XII) (SytXII)
Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 12 differs from classical synaptotagmins in that it does not bind Ca(2+). In synaptic vesicles, synaptotagmin-12 forms a complex with synaptotagmin-1 that prevents synaptotagmin-1 from interacting with SNARE complexes (Maximov A. et al., 2007).
ICC, IHC, WB
45 kDa (plus others)
Fusion protein amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12 (also known as Syt12, Synaptotagmin XII, SytXII, Synaptotagmin-related gene 1 protein, Srg1 and Sytr1, accession number Q920N7); Rat: 100% identity (88/88 amino acids identical); Human: 95% identity (84/88 amino acids identical); Greater than 30% identity with Synaptotagmin-6 (and other Synaptotagmins)
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Does not cross-react with Synaptotagmin-6 (or other Synaptotagmins)
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.