Primary Antibodies

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Adult rat brain membrane immunoblot.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or extracts of COS cells transiently transfected with Myc- tagged LRRTM1, LRRTM2 or untagged Kv2.1 plasmid probed with N209C/35 (left), K89/34 (middle) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Adult rat brain membrane immunoblotRepresentative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat. 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/3 (left) and N86/8 (right) TC supe.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/3 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing staining of GFAP. The sections were stained with Antibodies Incorporated anti-GFAP antibody (visualized in green)  (Cat. 75-240) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
NeuroMab Anti-GFAP Antibody
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Adult rat brain membrane immunoblot.
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N202/7 (left) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Adult rat brain membrane immunoblot.
KC rabbit anti- Kv2.1 Brain samples courtesy of Albee Messing, UW- Madison.Immunofluorescence of GFAP WT and KO mouse hippocampus with N206B/9 Brain samples courtesy of Albee Messing, UW- Madison.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ZNF746, V5-tagged ZNF398, V5-tagged ZNF783 or untagged Kv2.1 plasmid and probed with N196/16 TC supe (left), N86/8 TC supe (middle) or Rabbit anti-V5.
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged QKI-5, QKI- 6, QKI-7 and QKI-7b and untagged Kv2.1 plasmids and probed with N195A/16 TC supe (left) and mouse anti- Flag (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gammaprotocadherin-C4 or untagged Kv2.1 plasmid and probed with N193A/13 (left) and N86/8 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged Frataxin and untagged Kv2.1 plasmids and probed with N191/7 (left), 19E10 (middle) and N52A/42 (right) TC supe.
Adult rat striatum immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Immunoblot versus adult rat brain membranes (RBM), and adult mouse hippocampal membranes (MHM) from SAP102 knockout and WT mice (mouse samples courtesy of Dr. Richard Huganir).
Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunolabeling of postnatal 14 mouse CA3 specifically labeling PSD93 (Cat. 75-057, 1:400, green) and Ankyrin G(red). DNA labeled with DAPI. Mice were transcardially perfused with ice-cold 4% paraformaldehyde in PBS, then brain removed and postfixed overnight. The 50uM slices were floating sections. Objective 25x, scale bar = 20um. Images kindly provided by Jorge Ramirez-Franco and Oussama El Far, Aix-Marseille Université and INSERM. Immunoblot against crude membranes from whole adult rat brain (RBM) or hippocampi (MHM) from adult PSD-93/Chapsyn-110 knockout (KO) or wild-type (WT) mice. Mouse samples courtesy of Richard Huganir (Johns Hopkins University).
Immunoblot against crude membranes from whole adult mouse brain (MBM) or rat brain (RBM) and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N18/28 (left) or N52A/42 (right) TC supe.Immunofluorescence staining of 14 DIV cultured rat hippocampal neurons.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.

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