Primary Antibodies

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Immunoblot versus crude membrane preparations from adult rat brain (RBM) and Brevican wild-type (WT) and knockout (KO) mice and probed with N294A/6 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Matthew Rasband, Baylor College of Medicine.
Immunofluorescence against SUR1 WT (left) and null mutant (right) mouse striatum and stained with N289/16 (green) and commercial antibody against Tyrosine Hydroxylase (blue). Images courtesy of Paul Witkovsky, Jyoti Patel and Margaret Rice, New York University Medical Center.Adult rat hippocampus immunohistochemistry (with antigen retrieval via Na citrate pretreatment).
Immunohistochemistry with antigen retrieval via citrate pretreatment.Adult rat hippocampus immunohistochemistry under standard protocol.
Immunofluorescence staining of zebrafish sections at 5 days post- fertilization with N286/74 (green) and Synapsin 1/2 rabbit polyclonal (red). Left: optic tectum (TeO), inner plexiform layer (asterisk) and photoreceptor layer (arrow). Right: synapse-rich axonal projections in optic tectum. Image courtesy of Philip Washbourne (University of Oregon).Immunoblot versus homogenates from adult zebrafish muscle (Non- neuronal), brain (Neuronal) and purified brain postsynaptic densities (PSD) probed with N286/74 TC supe. Image courtesy of Philip Washbourne (University of Oregon).
Coronal sections of rat cerebellum, stained with human patient autoantibodies (E, red) and anti-vGlut1 (F, green labeling, Cat no 75-066, 1:300). Colocalization was not observed when mixing the anti-VGlut1 with the autoantibodies (merge G, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228HEK293T transfectants expressing SLITRK1 or its mutants were co-cultured with hippocampal neurons. Two days after co-culture, induced pre-synapses were quantified through VGAT or VGLUT1 immunostaining and GFP. Image from publication CC-BY-4.0. PMID:36085162
NeuroMab Anti-VGlut1 Antibody
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Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N29/29 (left) or N52A/42 (right) TC supe.Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N29/29 (left) and N52A/42 (right) TC supe.
NeuroMab Anti-VGlut2 Antibody
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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N278/19 (left) and N86/8 (right).Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N277/7 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N276A/15 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right).Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ASIC1, ASIC2 or untagged Kv2.1 plasmid and probed with N271/44 (left) or N86/8 (right) TC supe.Adult rat brain immunohistochemistry.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or wild-type (WT) or KA2 knockout (KO) mice probed with N279B/27 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Anis Contractor (Northwestern University.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS-1 cells transiently transfected with KCNQ1, KCNQ2, KCNQ3, KCNQ4, KCNQ5 or Kv2.1 plasmids and probed with pure N26A/23.
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N270/47 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin plasmid and probed with N269/73 (left) and N86/8 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N274/8 (left) and N86/8 (right).
Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Clcn3 KO mouse brains probed with N258/5 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Fred Lamb (University of Iowa.
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with untagged Cav1.2, Cav1.3 or Kv2.1 plasmids and probed with N263/31 (left) and L57/46 (right) TC supe.TIRF images (top row), super-resolution GSD localization maps (middle row) and binarized images (bottom row) of immunostained CaV1.2 (Cat. 75-257, 1:200) channels and β1ARs in representative fixed adult ventricular myocytes isolated from WT mice under control (A) or ISO-stimulated conditions (B). Merged two-channel image showing relative distributions of CaV1.2 and β1ARs is shown in the third column with the colocalized binarized image. Image from publication CC-BY-4.0. PMID:37438479
Immunoblot against adult rat brain membrane (RBM) or low- speed pellet (RBLSP) fractions denatured at 95oC for 5 min or 37oC for 15 min and probed with N253/32 (left) and K58/35 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N25/35 (left) or N52A/42 (right) TC supe.
Immunoblot against bacterially expressed Thioredoxin (TRX) and/or 6xHistidine (His) tagged fusion proteins.
Immunolabeling of mouse skeletal muscle staining NaV 1.4 (Cat. 75-424, 1:1000, red) and acetylcholine receptor (red). The frozen tissue section was fixed with 4% PFA for 10 minutes and mounted before staining. Image kindly provided by Chengjie Xie and Lauren Fish, Brown University.
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged TARPGamma2/3/4/8 or untagged Kv2.1 plasmid and probed with N245/36 (left) and N86/8 (right) TC supe.

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