Primary Antibodies

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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Gamma- protocadherin-C3 and –A3 and untagged Kv2.1 plasmids and probed with N174B/27 (left) and N159/5 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged Tafazzin or untagged Kv2.1 plasmid and probed with N173B/13 (left) and 12CA5 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged INPP4b or untagged Kv2.1 plasmid and probed with N171/17 (left) and N86/8 (right) TC supe.Immunoblot of Inpp4b+/+ or Inpp4b−/− mouse embryonic fibroblasts treated with vehicle or apilimod 10 nM for 48 h and assessed for protein levels of Inpp4b, LC3, and Beta actin as loading control. Image from publication CC-BY-4.0. PMID: 35760104
Immunoblot versus adult rat brain membranes (RBM) and whole brain homogenates from S100A5-tauGFP (targeted deletion of S100A5 and replaced with coding region of tau fused to GFP) heterozygote (Het) and KO mice and probed with N176A/35 TC supe (left) and rabbit anti-S100A5 antibody (right). Samples courtesy of Tim McClintock, University of Kentucky.
Adult rat hippocampus immunohistochemistry.Fluorescence images showing the specific lableling of Kv 3.1 (Cat. 75-041) identifying Ascl1SA6/Bcl2 mouse cortical glia converting into induced neurons. Image provided by publication. PMID: 39454007
Adult rat sciatic nerve immuno-fluorescence staining: paranodes labeled with Caspr (green) surround nodes of Ranvier labeled with Beta-IV spectrin (blue) and N168/6 (red). Images courtesy of Shelly Buffington and Matt Rasband (Baylor College of Medicine).Adult rat brain immunohistochemistry N168/6 staining in striatal efferents in globus pallidus.
Immunoblot versus adult rat brain membranes (RBM) and extracts of embryonic stem (ES) cells from wild-type (WT) and Dicer knockout (KO) mice probed with N167/7 (left) and N52A/42 (right, loading control) TC supe. Mouse ES cell samples courtesy of Chryssa Kanellopoulou and David Livingston, Harvard.
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged Kv2.1, GFP-tagged OCRL or RFP-tagged INPP5b plasmids and probed with N166A/26 (left) and another cross-reacting mouse OCRL antibody (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Neurexin-1-Alpha, Neurexin-1- Beta or untagged Kv2.1 plasmid and probed with N170A/1 TC supe (left) and rabbit anti-Flag (right).
Adult rat brain membrane immunoblot.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N161/20 TC supe (left) and a mouse loading control (right).
Adult rat brain immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged SynDIG3 or untagged Kv2.1 plasmid and probed with N160/21 (left) and K89/41 (right) TC supe.
Immunostaining of spinal cord samples from WT and Gamma-protocadherin KO mice with N159/5 TC supe. Data courtesy of Joshua Weiner, University of Iowa.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc- tagged Uncx and untagged Kv2.1 plasmids and probed with N155/9 (left) and 19E10 (right) TC supes.Immunofluorescence of embryonic day 8.5 mouse embryo whole mounts showing posterior staining of somites (arrows). Data courtesy of Rieko Ajima and Terry Yamaguchi, Cancer and Developmental Biology Laboratory, National Cancer Institute.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged JMJD2A and untagged Kv2.1 plasmids and probed with N154/32 (left) and K89/41 (right).
Adult rat hippocampus immunohistochemistry.Immunoblot versus crude cardiac mitochondrial preps from WT, Mfn1 KO or Mfn2 KO mice and probed with N153/5 TC supe (top) and Porin antibody (bottom). Data courtesy of Dr. Suzanne Hoppins, Dr. Laura Lackner and Dr. Jodi Nunnari, UCDavis.
Adult rat hippocampus immunohistochemistry (with antigen retrieval via pepsin pretreatment.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with GFP-tagged Doc2b or untagged Kv2.1 plasmids and probed with N150/21 (left), N86/8 (middle) and K89/41 (right).
Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).
Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).Transfected HEK293 cell immunofluorescence staining N15/39=green, DAPI=blue.
Transfected HEK293 cell immunofluorescence staining N15/4 TC supe =green, DAPI=blue.Transfected cell immunoblot. Extracts of COS-1 cells transiently transfected with rat TrpV3 (left lane) or rat Kv2.1 (right lane) plasmids and probed with K89/34 TC supe (left panel) or pure N15/4 (right panel).
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.

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