Primary Antibodies

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Immunostaining of mouse brain cortex identifying the calcium channels along axons stained with Anti-Cav1.3 Ca2+ Channel Antibody (Cat. 75-080, 1:500, green) and nuclei are labeled with DAPI (blue). Image kindly provided by Kaveh Moradi, Virginia Commonwealth University.Adult rat hippocampus immunohistochemistry.
Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/60 (red) and Hoechst nuclear stain (blue.Immunoblot against extracts of COS cells transiently transfected with GFP-tagged Kv2.2long, Myc-tagged Kv2.2short or untagged Kv2.1 plasmid probed with N372B/60 (left), N372C/51 (middle) or N52A/42 (right) TC supe.
Immunofluorescence staining of wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372C/51 (red) and Hoechst stain (blue).Adult rat brain immunohistochemistry.
Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/1 (red) and Hoechst nuclear stain (blue).Adult rat whole brain immunohistochemistry.
Distribution of SHANK3 immuno-positive cells in different regions of the adult mouse cerebral cortex. N, O: High-magnification views of SHANK3-positive cells in the RSPV region (N) and hippocampal region (O) as shown in image M. Green, SHANK3-positive cells (Cat. 75-344, 1:200); blue, cell nuclei. Scale bars, 300 μm (M); 100 μm (N, O). Image from publication, CC-BY-4.0. PMID: 39693031.Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).
adult rat Whole brain immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA- tagged Kir6.2 or untagged Kv2.1 plasmid probed with N366/60 (left) or N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
Immunofluorescence staining of adult spinal cord from Bral1 wild-type (WT).Knockout (KO) mice with N364/10 (green) and bIV- spectrin rabbit polyclonal (red). Images courtesy of Kae-Jiun Chang and Matt Rasband (Baylor College of Medicine).
Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA-tagged Kir6.2 or untagged Kv2.1 plasmid probed with N363/71 (left) or N52A/42 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Shank1, Shank2, Shank3 or untagged Kv2.1 plasmid and probed with N367/51 (left) or N23B/49 (right) TC supe.
Immunoblot against adult rat brain membranes (RBM) and membranes from SVOP knockout (KO) and wild-type (WT) mice probed with N356/23 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Jia Yao and Sandra Bajjalieh (University of Washington.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged ATF4 or untagged ATF5 or Kv2.1 plasmid and probed with N360A/24 TC supe (left), Rabbit anti-ATF5 (middle) or K89/34 TC supe (right).
Transfected cell immunofluorescence: COS cells expressing Myc-tagged Flrt3. Red = Myc, Green = N353/13, Blue = Hoechst nuclear stain.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmid and probed with N348/82 (left) and K89/34 (right) TC supe.
Immunofluorescence staining of adult hippocampus (sp = stratum pyrimidale, sl = stratum lucidum) from Stonin-2 wild-type (WT) and knockout (KO) mice with N346/9 (red) and K89/34 (green). Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Stonin-2 KO mouse brains probed with N346/9 (left), K89/34 (middle) and N52A/42 (right) TC supes. Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag- tagged REEP1, REEP2 or untagged Kv2.1 plasmid and probed with N345/51 (left) and K89/34 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Foxi2 or untagged Kv2.1 plasmid and probed with N349/96 TC supe (left), Rabbit anti-Flag (middle) or N52A/42 TC supe (right.
Immunofluorescence staining of a node of Ranvier in adult rat sciatic nerve with N343/26 (green) and bIV-spectrin rabbit polyclonal (red). Image courtesy of Kae-Jiun Chang and Matt Rasband (Baylor College of Medicine.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of CV-1 fibroblast cells transfected with VGlut3 (+) or left untransfected (-) and probed with N34/34 TC supe. Image courtesy of Jeff Erickson, Louisiana State University Health Sciences Center.
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged LRRK1, LRRK2 or untagged Kv2.1 plasmid and probed with N341/12 (left) or N241A/34 (right) TC supe.Immunoblot versus LRRK1-immunoprecipitated extracts from WT and LRRK1 KO mouse heart probed with N341/12 TC supe. Data courtesy of Paul Davies and Dario Alessi (University of Dundee, Scotland, UK.
Adult rat hippocampus immunohistochemistry.Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged Alpha-2C and Alpha-2A adrenergic receptor or untagged Kv2.1 plasmid and probed with N330A/80 TC supe.

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