Polyclonal Antibodies

1041 products

Polyclonal antibodies recognize multiple epitopes on the same target protein and therefore can offer strong signal-to-noise as the binding at multiple sites allows for many detection molecules to bind.

Antibodies Inc is proud to offer several product lines and flavors of polyclonal antibodies. Rabbit polyclonals are an industry standard for polyclonal antibodies because the medium size of rabbits allows for larger pools of serum. With a focus in both neuroscience and cancer research, our PhosphoSolutions line specializes in phosphospecific rabbit polyclonal antibodies.

Chicken polyclonals have grown in popularity especially when performing multiplex analysis using secondary antibodies. We offer the complete product line from Aves Labs: a pioneer in the use of chickens for the production of polyclonal research antibodies. Evolutionarily removed from mammals, chickens mount a vigorous immune response to mammalian gene products, resulting in high-affinity IgY antibodies. A unique species like chicken is also perfect for multiplexing applications, with low cross-reactivity to other species.

Antibodies Inc offers polyclonal centromere antibodies which are purified IgG derived from human anti-centromere positive serum (CREST patient serum). Useful as diagnostic markers in assays such as IHC and ICC, as well as controls in ANA diagnostics, they help identify autoimmune reactions.

Showing 169 - 192 of 1041 products

Biosensis Anti-Myelin basic protein, degraded (dMBP) Antibody

$337

Biosensis Anti-Vanilloid receptor-like protein 1 (VRL-1) Antibody

$337

Biosensis Anti-Alpha-synuclein Antibody

$337

Immunofluorescent staining of mock- or 50 µM chloroquine-treated HeLa cells using 2 µg/mL chicken anti-SQSTM1 (p62) antibody (green). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). Anti-SQSTM1 (p62) specifically stains the autophagosomes in HeLa cells. Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.

Western blotting of mock- or 50 µM chloroquine-treated HeLa cell lysates with Aves Labs chicken anti-SQSTM1 (p62) antibody at 1:5000 (0.04 µg/ml) and 1:10000 (0.02µg/ml) dilutions and detected with goat anti-chicken HRP. Aves Labs Chicken anti-SQSTM1 (p62) recognizes endogenous SQSTM1 (p62) in HeLa cells at ~62 kDa. Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.

Aves Labs Anti-SQSTM1 Antibody

From $144

Immunofluorescent staining of MCF7 cells using 1 µg/mL chicken anti-HB9 (MNX1) antibody (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with ICT's Fluoroshield mounting medium (Cat. AR-6500). Anti-HB9/MNX1 specifically stains the nucleus of MCF7 cells.

Western blotting of MCF7 cell lysates with chicken anti-HB9 (MNX1) antibody at various concentrations and detected with anti-chicken HRP. Chicken anti-HB9/MNX1 recognizes endogenous MNX1 in MCF7 cell lysates at ~50 kDa.

Aves Labs Anti-MNX1/HB9 Antibody

From $144

Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).

Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

PhosphoSolutions Anti-WAVE1 (N-terminal region) Antibody

$348

Western blot image of GST-recombinant human VEGFR-1 (89 kDa), VEGFR-2 (110 kDa), and VEGFR-3 (86 kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801, conserved site), and anti-VEGFR-3 (a.a. 1285-1298).

Left: Western blot image of HUVEC cells untreated (-) or treated with pervanadate (1 mM) for 30 min. (+). Right: Western blot image of GST-recombinant VEGFR-2 kinase without (-) or with (+) akaline phosphatase treatment. Both sets of blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317) or anti-VEGFR-2 (Tyr-801).

PhosphoSolutions Anti-VEGFR-2 (Tyr-801) [conserved site], Phosphospecific Antibody

$348

Western blot analysis of 250 ng/lane of a1-Tubulin unphosphorylated (lanes 1, 3, & 5) or phosphorylated at Ser-165 with PKCα (lanes 2, 4, & 6). The blots were probed with anti-α6-Tubulin (a.a. 160-169) (TP4281; lanes 1 & 2), anti-α6-Tubulin (Ser-165) (TP4131; lanes 3 & 4), and anti-α-Tubulin (C-terminus) (TM4111; lanes 5 & 6).

PhosphoSolutions Anti-α6-Tubulin (Ser-165), Phosphospecific Antibody

$348

Western blot analysis of adult mouse diaphram treated with thapsigargin for 0, 6, 12, or 24 hours to induce cleavage of αII-spectrin from 250 kDa to 136 kDa. The blot was probed with rabbit polyclonal αII-spectrin (a.a. 1171-1176) cleavage-specific antibody at a dilution of 1:1,000 (Image provided by Dr. Leigh Ann Callahan, Department of Internal Medicine, University of Kentucky.)

PhosphoSolutions Anti-αII-Spectrin , cleavage-specific Antibody

$348

Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).

Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-118)
phospho-specific (SP5521). The antibody was detected using goat anti-rabbit DyLight® 594.

PhosphoSolutions Anti-Sox2 (Thr-118), Phosphospecific Antibody

$348

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to alkaline phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Tyr-536) antibody (lanes 3-5). The SHP1 (Tyr-536) antibody was used in the presence of phospho-SHP1 (Tyr-536) peptide (lane 5).

PhosphoSolutions Anti-SHP1 (Tyr-536), Phosphospecific Antibody

$348

Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B (Tyr-152) (lanes 3 & 4).

PhosphoSolutions Anti-PTP1B (Tyr-152), Phosphospecific Antibody

$348

Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and rabbit polyclonal anti-phospho-Paxillin (Tyr-118) (PP4501) (lanes 3 & 4).

PhosphoSolutions Anti-Paxillin (Tyr-118), Phosphospecific Antibody

$348

A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38α (a.a. 319-328) (lanes 1 & 2) or anti-p38α (Tyr-323) (lanes 3 & 4).

Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

PhosphoSolutions Anti-p38α MAP Kinase (Tyr-323), Phosphospecific Antibody

$348

Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).

Formalin fixed, citric acid treated parafin sections of adult Rat striatum. Sections were probed with anti-nNOS (NP2141) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

PhosphoSolutions Anti-nNOS (C-terminal region) Antibody

$292

Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1µg/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).

Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-iNOS (Tyr-1055) [conserved site], Phosphospecific Antibody

$348

Western blot image of human A431 cells stimulated with calyculin A (100 nM, 30 min). The blots were untreated (lanes 1, 3 & 5) or treated with lambda phosphatase (lanes 2, 4 & 6), and probed with rabbit polyclonals Myosin IIA Heavy Chain (a.a. 1936-1950) (lanes 1 & 2), Myosin IIA Heavy Chain (Ser-1803), phospho-specific (lanes 3 & 4) or Myosin IIA Heavy Chain (Ser-1916) phospho-specific (lanes 5 & 6).

PhosphoSolutions Anti-Myosin IIA Heavy Chain (Ser-1916), Phosphospecific Antibody

$348

Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).

Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Myosin IIA Heavy Chain (a.a. 1936-1950), then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3795).

PhosphoSolutions Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody

$292

Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).

PhosphoSolutions Anti-Histone H2B (N-terminal region) Antibody

$348

Immunocytochemical labeling of Histone H2B in methanol and acetone fixed rat A7r5 cells. The cells were labeled with rabbit polyclonal Histone H2B (C-terminus) antibody (HP4291), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-Histone H2B (C-terminus) Antibody

$348

Western blot image of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then were probed with anti-EGFR (a.a. 961-972) (lanes 1 & 2), anti-EGFR (Ser-967) (lanes 3 & 4), and anti-EGFR (Ser-1142) (lanes 5 & 6).

Immunocytochemical labeling in A431 cells untreated or treated with Calyculin A (50 nM) for 15 min. Cells were labeled with anti-EGFR (a.a. 961-972) or anti-EGFR (Ser-1142) antibodies. The specificity of labeling was demonstrated for each antibody by blocking the signals with their respective blocking peptides, EGFR (a.a. 961-972) (EX1875) and phospho-EGFR (Ser-1142) (EX1935).

PhosphoSolutions Anti-EGFR (N-terminal region) Antibody

$292

Recombinant human eEF2K untreated (lanes 1 & 4), auto-phosphorylated in the presence of Ca2+ and Calmodulin (lanes 2 & 5), and dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-500) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)

Western blot image of C2C12 untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-eEF2K (N-terminus) (lanes 1 & 2) and anti-eEF2K (Ser-500) (lanes 3 & 4).

PhosphoSolutions Anti-eEF2K (Ser-500), Phosphospecific Antibody

$348

Recombinant human eEF2K untreated (lanes 1 and 4) and phosphorylated with p38 kinase in vitro (lanes 2 & 5). After in vitro reaction, the eEF2K was dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-359) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)

PhosphoSolutions Anti-eEF2K (Ser-359), Phosphospecific Antibody

$348

Western blot analysis of anti-γ-Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with γ-Catenin (a.a. 545-555), γ-Catenin (Tyr-550) or γ-Catenin (C-terminal) antibodies. The anti-γ-Catenin (Tyr-550) was used in the presence of γ-Catenin (Tyr-550) (lane 5) or γ-Catenin (Tyr-644) (lane 6) peptides.

Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal γ-Catenin (CM1111) or rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.

PhosphoSolutions Anti-γ-Catenin (Tyr-550), Phosphospecific Antibody

$348

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Polyclonal Antibodies

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