Polyclonal Antibodies

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Western blot of mouse whole brain lysate showing specific immunolabeling of the ~28 kDa TREM2 protein.Immunofluoresence of FLAG-tagged TREM2 transfected COS cells showing TREM2 (cat. 2006-TREM2, 1:150) staining in red and FLAG staining in green causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.
PhosphoSolutions Anti-TREM2 Antibody
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Western blot of rat hippocampal lysate showing specific labeling of the ~38 kDa Synaptophysin2 protein. Immunostaining of 40DIV cultured rat cortical neurons showing punctate labeling of synaptophysin (cat. 1950-SNT2, red, 1:100). The blue is staining nuclear DNA. Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synaptophysin 2 Antibody
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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 27 kDa Olig1 protein.Immunostaining of primary rat oligodendrocytes using anti-Olig1 (cat. 1537-OLIG1, red, 1:100).
PhosphoSolutions Anti-Olig1 Antibody
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Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells. Immunolabeling of HEK 293 cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-UCHL1 antibody (cat. 2060-UCHL1, green, 1:500).
PhosphoSolutions Anti-NSE (Neuron Specific Enolase) Antibody
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Western blot of 10 µg of HEK 293 cells specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the C2’ splice variant insert (lanes 3 and 4). 1) No NR1 expression; 2) NR1 subunit containing only the C2 Insert; 3) NR1 subunit containing the C1 and C2' Insert; 4) NR1 subunit containing the N1 and C2' Insert.
PhosphoSolutions Anti-NMDA NR1 Subunit, Splice Variant C2' Antibody
$445
Western blot of 10 µg of HEK 293 cells showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the C1 splice variant insert (in lane 3). 1) HEK cells without NR1 expression; 2) NR1 subunit containing only the C2 Insert; 3) NR1 subunit containing the C1 and C2' Insert; 4) NR1 subunit containing the N1 and C2' insert.
PhosphoSolutions Anti-NMDA NR1 Subunit, Splice Variant C1 Antibody
$445
Western blot of mouse habenula lysate showing specific immunolabeling of the ~52 kDa nAChRβ2 protein.
PhosphoSolutions Anti-Nicotinic Acetylcholine Receptor (nAChR) β2 Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN (cat. 583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
PhosphoSolutions Anti-Neurofilament NF-L Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
PhosphoSolutions Anti-Neurofilament NF-H Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~115 kDa APP protein.
PhosphoSolutions Anti-Amyloid Precursor Protein Antibody
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Western blot of HeLa lysate showing specific immunolabeling of the ~47 kDa Mnk2b isoform in the first lane (-). Specificity is shown in the third lane (Mnk2b) where immunolabeling is blocked by preadsorption of the Mnk2b peptide used as the antigen, but not by the Mnk2a peptide in the second lane.
PhosphoSolutions Anti-Mnk2b Antibody
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Western blot of HeLa lysate showing specific immunolabeling of the ~52 kDa Mnk2a isoform in the third lane (-). Specificity is shown in the second lane (Mnk2a) where immunolabeling is blocked by preadsorption of the Mnk2a peptide used as the antigen, but not by the Mnk2b peptide in the first lane.
PhosphoSolutions Anti-Mnk2a Antibody
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Western blot of mouse whole brain lysate showing specific immunolabeling of the ~38 kDa Mff protein.
PhosphoSolutions Anti-Mitochondrial Fission Factor (Mff) Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~15 kDa alpha synuclein protein phosphorylated at Ser129 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Alpha Synuclein (Ser129) Antibody
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Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
PhosphoSolutions Anti-ERK/MAPK (Thr202/Tyr204) Antibody
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Immunostaining of a frozen section of paraformaldehyde-fixed mouse brain showing specific immunolabeling of DDC (1:500) in red and fluorescent Nissl (blue). Photo courtesy of Tom Finger, University of Colorado School of Medicine. Western blot of rat adrenal medulla showing specific immunolabeling of the ~55 kDa DDC protein.
PhosphoSolutions Anti-DOPA Decarboxylase Antibody
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Western blot of rat kidney lysate showing specific immunolabeling of the ~24 kDa TFAM protein. Western blot of 143B cells showing specific immunolabeling human TFAM (top row). Knockout validation is demonstrated in lane 2 where cells transfected with altered TFAM via GeneSwap show no immunolabeling. Image from publication CC-BY-4.0. PMID: 35883613
PhosphoSolutions Anti-TFAM (Transcription Factor A, Mitochondrial) Antibody
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Immunofluorescent staining of HeLa cells using 1 µg/mL Aves Labs chicken anti-GM130 (GOLGA2) (Cat No. GOLGA2-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. No. AR-6501). Anti-GM130 specifically stains the Golgi apparatus in HeLa cells. ​Western blotting of various cell lysates with Aves Labs chicken anti-GM130 (GOLGA2) antibody (0.5 µg/ml) and detected with anti-chicken HRP. Aves Labs Chicken anti-GM130 recognizes endogenous GM130 in all the cell lysates at ~130 kDa.
Aves Labs Anti-GOLGA2 (GM130) Antibody
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BCAT1-3 1782 × 452px Staining of COS7 cells overexpressing FLAG-tagged beta-catenin with: chicken anti-beta catenin antibody BCAT (left panel in green) showing both endogenous staining of beta catenin in COS7 cells and staining of overexpressed nuclear beta-catenin (white arrow), anti-FLAG staining of overexpressed nuclear beta-catenin (middle panel in red), and overlay (right panel) with DAPI staining of nuclei to show all cells.Staining of paraffin wax mouse lung sections using beta-catenin antibody (Cat. No. BCAT, 1:100 dilution) with antigen retrieval and biotinylated secondary antibodies plus streptavidin. Image courtesy of Kuan Zhang (UCSF).
Aves Labs Anti-Beta-Catenin Antibody
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Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of NCAM1. The sections were stained with anti-NCAM1 chicken polyclonal antibody (visualized in green) at 1 µg/mL (1:200) dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat no. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Magnification 100X.Western blotting of rat brain homogenate with chicken anti-NCAM1 antibody and detected with goat anti-chicken HRP. Chicken anti-NCAM1 recognizes endogenous NCAM1 in rat brain homogenate at ~180 kDa.
Aves Labs Anti-NCAM1 Antibody
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Immunofluorescent staining of mock- or 50 µM chloroquine-treated HeLa cells using 2 µg/mL chicken anti-SQSTM1 (p62) antibody (green). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). Anti-SQSTM1 (p62) specifically stains the autophagosomes in HeLa cells. ​Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.Western blotting of mock- or 50 µM chloroquine-treated HeLa cell lysates with Aves Labs chicken anti-SQSTM1 (p62) antibody at 1:5000 (0.04 µg/ml) and 1:10000 (0.02µg/ml) dilutions and detected with goat anti-chicken HRP. Aves Labs Chicken anti-SQSTM1 (p62) recognizes endogenous SQSTM1 (p62) in HeLa cells at ~62 kDa. Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.
Aves Labs Anti-SQSTM1 Antibody
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Immunofluoresence of COS7 cells expressing mScarlet3 using chicken anti-mScarlet3 antibody (green) and showing mScarlet3 autofluorescence (red). The cells were mounted with ICT's Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from chicken anti-mScarlet3 antibody and mScarlet3 autofluoresence in transfected cells.Western blotting of mock or mScarlet3 transfected COS-7 cell lysates with chicken anti-mScarlet3 antibody (0.5 ug/ml) and detected with anti-chicken HRP. Chicken anti-mScarlet3 recognizes exogenous mScarlet3 in COS-7 cells.​
Aves Labs Anti-mScarlet3 Antibody
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Immunofluorescent staining of MCF7 cells using 1 µg/mL chicken anti-HB9 (MNX1) antibody (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with ICT's Fluoroshield mounting medium (Cat no. AR-6500). Anti-HB9/MNX1 specifically stains the nucleus of MCF7 cells.​Western blotting of MCF7 cell lysates with chicken anti-HB9 (MNX1) antibody at various concentrations and detected with anti-chicken HRP. Chicken anti-HB9/MNX1 recognizes endogenous MNX1 in MCF7 cell lysates at ~50 kDa.
Aves Labs Anti-MNX1/HB9 Antibody
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Immunofluorescence staining of melanopsin in mouse retinal tissue using mOPN4 chicken antibody (Cat No. mOPN4-0100).
Aves Labs Anti-mOPN4 (Melanopsin) Antibody
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