Monoclonal Antibodies

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Adult rat brain membrane immunoblot.
Adult rat optic nerve immunofluorescence staining L11A/41 (5 μg/ml)=red Rabbit anti-Caspr=green Image courtesy of Dr. Matthew Rasband.Adult rat brain membrane immunoblot.
Adult rat brain striatum and globus pallidus immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Adult rat olfactory bulb immunohistochemistry.Adult rat cerebellum immunohistochemistry.
Adult rat brain immunohistochemistry.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and KChIP3 KO mouse brains probed with K66/38 (left) and K55/82 (right) TC supe. Mouse samples courtesy of Takeshi Sakurai and Joseph Buxbaum (Mount Sinai School of Medicine).
Immunofluorescence staining transiently Kv1.6-transfected COS cells.Immunoblot on adult rat brain membrane (RBM) and lysates from Kv1.6-transfected (COS+) and untransfected (COS-) COS cells.
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with 8G10 (left), N241A/34 (middle) and K89/34 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson, Johns Hopkins University
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine (PM3801) and rabbit polyclonal anti-Phosphoserine/threonine (PP2551), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.

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