Anti-JMJD2A Antibody (N154/32)

Name: Anti-JMJD2A Antibody
Brand: NeuroMab
SKU: 75-189
Price: 337.0 USD
Availability: InStock

Our mouse JMJD2A monoclonal antibody from NeuroMab is produced in-house from clone N154/32. It detects human JMJD2A and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged JMJD2A and untagged Kv2.1 plasmids and probed with N154/32 (left) and K89/41 (right).

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Species Reactivity

Human

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-189

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

JMJD2A

Scientific Background

Lysine Demethylase 4A is encoded by the gene KDM4A. KDM4A is a member of the JHDM3 histone demethylase family. KDM4A is a nuclear protein that contains a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. KDM4A functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. KDM4A is expressed is several organs with the highest expression in the lungs. Diseases associated with KDM4A include Attention Deficit-Hyperactivity Disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N154/32

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

KDM4A JHDM3A JMJD2 JMJD2A KIAA0677

Molecular Weight

120 kDa

Immunogen

Fusion protein amino acids 568-674 of human JMJD2A (accession number O75164) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human

Antibody Registry ID

AB_10671303

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.117

Conjugate

Unconjugated

Specificity

Does not cross-react with JMJD2C

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

JHDM3A; Lysine-specific demethylase 4A; JMJD2A; EC 1.14.11.-; TDRD14A; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A; lysine (K-specific demethylase 4A; tudor domain containing 14A

UniProt ID

O75164
UniProt Summary: Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Also able to demethylate histone H1-4 methylated at 'Lys-26' (H1.4K26me1, H1.4K26me2 and H1.4K26me3). Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.

NCBI Gene ID

9682

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Anti-JMJD2A Antibody (N154/32)

SKU: 75-189

Product Details

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