Anti-GABA-B-R1 Antibody (N93A/49)

Name: Anti-GABA-B-R1 Antibody
Brand: NeuroMab
SKU: 75-183
Price: 337.0 USD
Availability: InStock

Our mouse GABA(B)R1 monoclonal antibody from NeuroMab is produced in-house from clone N93A/49. It detects chicken, human, mouse, rabbit, and rat GABA(B)R1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunoblot versus crude membranes made from adult rat brain (RBM) or GABABR1 wild-type (WT) or knockout (KO) mice probed with N93A/49 (left) or K89/34 (right) TC supe. Mouse brains courtesy of Martin Gassmann and Bernhard Bettler (University of Basel).

Adult rat hippocampus immunohistochemistry.

Immunoblot versus crude membranes made from adult rat brain (RBM) or GABABR1 wild-type (WT) or knockout (KO) mice probed with N93A/49 (left) or K89/34 (right) TC supe. Mouse brains courtesy of Martin Gassmann and Bernhard Bettler (University of Basel).

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rabbit, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-183

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABA(B)R1

Scientific Background

Gamma-aminobutyric acid type B receptor subunit 1 or GABA BR1 is encoded by the gene GABBR1. GABA BR1 is a subunit of the G-protein coupled receptor for the neurotransmitter GABA (gamma-aminobutyric acid). It will inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. GABA BR1 is highly expressed in many tissues including eye, atrium, ventricle, lung and colon. In brain, wide expression is seen with high levels in the cerebral cortical layers and hippocampus. Cellularly, it is found at the cell junction, synapse, the postsynaptic cell membrane and cytoplasmic vesicle membrane. Diseases associated with GABBR1 include Temporal Lobe Epilepsy and Persistent Vegetative State.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N93A/49

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Gabbr1

Molecular Weight

115 kDa

Immunogen

Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABABR1 (accession number Q9Z0U4) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Chicken, Human, Mouse, Rabbit, Rat

Antibody Registry ID

AB_2108180

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.111

Dilution Ranges

WB: 1:200
IHC: 1:500
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity against GABABR2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

dJ271M21.1.1; Gamma-aminobutyric acid type B receptor subunit 1; dJ271M21.1.2; GABA-B receptor 1; FLJ92613; GABA-B-R1; GABA-B receptor 1; GABA-BR1; GABA-B-R1; GABABR1; GABA-BR1; Gb1; GABAB R1; GABAB subunit 1c; GABABR1; GABBR1 3; GABBR1; GABR1_HUMAN; Gamma aminobutyric acid (GABA B receptor 1; Gamma-aminobutyric acid type B receptor subunit 1; Gb1; GPRC3A; Seven transmembrane helix receptor

UniProt ID

Q9Z0U4
UniProt Summary: Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.

NCBI Gene ID

81657

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Anti-GABA-B-R1 Antibody (N93A/49)

SKU: 75-183

Product Details

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